Biochemical Study On The Quality Of Different Soybean Cultivars

The accumulating variation of protein, fat, seed weight and the accumulating process of protein subunits of prolamine, albumin, gobulin and glufelin were analyzed in the different -period of the early and late-maturing varieties,and the relationship between variation and quality was discussed. The results indicated that (1) As to the early and late-maturing varieties, the rate that the net content of protein and fat increased appeared slowly first and fast afterwards, at last S-curve. (2) The sequence of times that storage protein and partial protein subunits synthetized and accumulated was different.(3) The quickly-increasing period of protein, fat, seed weight and the different protein subunits was in the whole accumulating procces. In addition, the content of seed protein, fat and soybean isoflavone were measured, prolamine, gobulin and glufelin in the seed storage protein were extracted in turn, and analyzed by SDS-PAGE. The results were as follows: (1) Among the varieties tested, 6 were high in protein, 4 were high in fat, 6 were high in isoflavone, 2 were high in protein and isoflavone, 1 was high in fat and isoflavone, 1 was high in protein, fat and isoflavone. (2) By SDS-PAGE of seed storage protein ,4 were found to be high quality in protein, 2 were special varieties for tohu and one for young soybean.(3) Measurement of protein, fat and isoflavone connected with SDS-PAGE can be a handy and quick method for finding varieties that had high quality in protein.