Dynamic Regularity Of IgA Content In Chickens' Mucosal Immune System After Newcastle Disease Vaccine Immunity

The experiment was conducted to study the dynamic regularity of IgA content in chickens' mucosal immune system after La sola vaccining. The changes of IgA content in immunized chickens' tear, harderian gland, tracheal fluid, bile, duodenum fluid, appendices amygdale fluid and serum were all measured. On the other hand, the changes of HI titer in immunized chickens' tear, tracheal fluid and serum were also measured in the experiment, therefore, the results of present experiment can provide more detailed reference data and the basis theories for further research of the immunoreaction mechanism and the clinic determination of Newcastle Disease.In the experiment, 160 chickens with similar health condition were regarded as the experimental animal, which were randomly divided into four groups, with 40 chickens in each one at 14 days old. One was the control group, the others were immunized with La sola vaccine by water, nose instillation and muscle injection respectively at 14 and 28 day old. The results can be concluded as following:Firstly, all immunity approaches could produced mucosal immunity antibody IgA and humoral immunity antibody IgG, which would be gradually increased along with times of immunity .Secondly, IgA contents had relation to the immunity approaches. In mucosal immunity approaches, such as water group, nose instillation group, the IgA content of chicken' different immunoreaction tissues, such as tear, harderian gland, tracheal fluid, bile, duodenum fluid, appendices amygdale fluid, serum, was higher than that inthe humoral immunity approaches, such as muscle injection group. So the mucosal immunity approaches was very important to improve immunity effect.Thirdly, IgA content in nose instillation group was higher than that in water group in present experiment.Finally, although the same immunity method was used, there was different in IgA content and the dynamic regularity of IgA content in chickens' different mucosal immune system, such as tear, harderian gland, tracheal fluid, bile, duodenum fluid, appendices amygdale fluid, serum .