Research On Variation Mechanism And Influence On It's Genome After Introducing λ DNA Into Chinese Spring

With Chinese spring and D4 stable variant produced by introducing λDNA into the Chinese spring for materials , the obvious differences could be found on type characters, cytology level, protein level and DNA level. The main results were as follow : 1. After introducing λDNA into Chinese spring , D0 generation showed no obvious variations of type characters , but D2 appeared distinct differences from D1. The variant characters, which included stalk rigidity,plant height,ear length,ear type,spikelet number,grain number of spike and wheat awn etc.,were obvious and stably hereditary in D4 generation.2. Many abnormalities in meiosis of pollen mother cell were observed among current generation after introducing lambda phage DNA into Chinese Spring。Abnormalities observed were as follow:chromosome laggard,chromosome bridge,micronucleolus ,asynchronous cell division and chromosome fragment etc. The possible reason that aroused these chromosome abnormalities was the intricate cytology changes owing to the chromosome recomposition after lambda was integrated into chromosomes of receptor.3. The result of esterase and peroxidase showed that there were evident differences in enzyme activity and enzyme bands between Chinese spring and D4 variant. The electrophoresis of HMW-GS implied that the main differences lied in 1 subunit and 7+9 subunit between Chinese spring and D4 variant.4. In the identification of variations, receptor and donor and variants by RAPD method, special bands could be found between variations and donor in 3 primers from 120 primers. The results suggested that λDNA fragmentswere introduced into receptor's cells nucleus and were integrated into receptor's genome...