Effect Of Growth Regulators On Plantlet Regeneration In Okra In Vitro

The aim of the present study was to investigate the possible involvement of growth regulator in plantlet regeneration of okra (Abelmoschus esculentus L). Hypocotyl, cotyledon and root explants of okra were cultured on Murashige and Skoog medium supplemented with different concentrations of various growth regulators, to evaluate the effect of growth regulators on in vitro shoot and root formation.The best shoot induction from cotyledon and hypocotyl was recorded on medium supplemented with 4 mg/1 BA and 0.1 mg/1 NAA. However, the highest root percentage and root number per explant were achieved with 1 mg/1 BA and 0.1 mg/1 NAA.Highest shoot formation from hypocotyl was found on MS medium supplemented with 4 mg/l kinetin and 0.lmg/l NAA and from cotyledon with 2 mg/l kinetin and 0.1mg/1 NAA. Kinetin showed a little influence on shoot regeneration when cotyledon and hypocotyl was used as explants. The regeneration of root could effectively occurred with 0.5-1 mg/1 kinetin. Compared to zeatin, BA and kinetin was found better in inducing shoot and root regeneration. No shoot initiation was observed on root explant tested in this present study.Effects of different concentrations of CPPU in combination with 0.1 mg/1 NAA on callus formation from hypocotyl, cotyledon and root was studied. The highest callus from root and hypocotyl was observed at 0.1 mg/1 NAA + 4 mg/1 CPPU. Whereas in cotyledon, highest callus weight was observed at 2 mg/1 CPPU + 0.1 mg/1 NAA.Influences of BA and NAA on shoot induction from callus were also investigated. The result showed that BA failed to induce shoot, even after several subculture times.