Examination Of Seed Purity In Rice Hybrid By SSR Method

Eighteen rice hybrids and their parents were chosen to identify the hybrids' purity with SSR markers and phenotype. The two methods of purity identification were compared in order to research the availability and accurateness of SSR marker identifying the rice seed's purity .The results as follows:1. Eighteen rice hybrid parents' DNA were amplified by twenty SSR markers, one or four SSR primers performed polymorphism between the double parents to average every hybrid.2. No significant difference was found between the average purity identified by SSR marker and the field experiment with 20 rice hybrids which were planted at Chendu in spring in 2001.3. In order to compare the difference between the two method strictly and estimate the availability and accurateness, the field experiment were be expanded two environment and three replication ,the SSR method also be expanded three replication. The results of correlation and regression analysis show that the correlation coefficient were 0.785**,0.645** and 0.668" respectively between the SSR marker and Hainan, SSR and Chendu, Hainan and Chendu.The average purity identified by experiment in Hainan and Chendu were chosed dependent variable(yi ,y2) and the average purity identified by SSR marker were chosed independent variable(x) , the regression equation were y1=32.70+66.4x and y2=31.80+67.4x.These results would be provided reference for the seed purity identification by SSR marker.In summary ,no difference founded in seed average purity identified by two methods of the SSR marker and phenotype. Field identification as the traditional method of seed purity identification may be substituted by SSR marker with the development of market economy, but some problem of SSR marker still be explored in practice.