Evaluation Of Genetic Diversity For Sheep Breeds By Microsatellite DNA

The 30 microsatellite loci are vised in this experiment to analysis the genetic diversity of six sheep breeds in order to offer basis of use and protection of sheep genetics.1 The 30 microsatellite loci which are selected from 41 published ones show polymorphism. We get 284 alleles.2 Calculating the allele frequency and the special allele frequency of 30 microsatellites loci by GENEPOP ,GDA ,and basing the allele frequency , we get the gene diversity of sheep population, PIC and number of effective alleles.The result are 0.4833-0.6465 , 0.5189~0.6491 , 2.639-4.354 respecially. There results show that the genetic variation is abundant in every breed.3 Coming out the total population gene diversity, subpopulation gene diversity and genetic differentiation coefficient, the average number of the three index is 0.6348,0.5603,0.1174 respectively. These resells show that the variation is 12% to total population variation.4 By GDA and DISPAN we get Dcoa , Dsand DA genetic distance which arealmost resemble in different breeds.5 We make NJ and UPGMA cluster analysis according to Ds, DA and Dcoa, The maps of cluster are devided into 4 subdivision: Sunite and Wuzhumuqin,Fine-wool sheep of Inner Monglia and Xiaoweihan sheep, Ningxiatan sheep, Wujiaoduosaite.