Application Of AFLP In Variety Identification And Relative Relations In Sugarcane

Twenty four sugarcane germplasms from different countries and regions were identified and classified by AFLP (Amplified Fragment Length Polymorphism) technology in this study. The different AFLP fingerprintings of 24 sugarcane germplasms obtained were used for genetic diversity and cluster analyses. The results showed that there were abundant genetic diversities among the germplasms, the highest rate of polymorphism bands was 52.6%, the lowest 11.5%, and the average 41.2%. The 24 varieties were classified to 5 categories with the Word's Minimum Variance Cluster Analysis (WMVCA). YUETANG76/329 and YUNZHE68/154 were classified into one category with a minimum variance of 0.0112. MANGGE was classified solely. The minimum variance of another varieties were between 0.02 and 0.05. T23 and T25 also were identified by AFLP in this research. The results showed that the relative relations of the two varieties are very close, which average polymorphism bands occupied 10.2% of the total polymorphism bands, and the genetic similarity was 0.897, and the genetic distance was 0.103. The results also showed that AFLP had high repetition and rich polymorphism, and should be a reliable DNA fingerprinting technology, which can be used in many fields of sugarcane germplasm research.The method of DNA extraction and purification adopted to sugarcane AFLP were built and performed. The key techniques of AFLP analysis system applied in sugarcane, and the reliability and safety of silver staining were also discussed.