The Investigation Of Glutamate Thalli Protein And Evaluation On Its Nutrient Value

In order to exploit new protein resources, this paper investigated the technology and the methods of collecting glutamate thalli protein and its ecnomic benefits and envorimental benefits of exploring glutamate thalli protein. The routine nutrients, true protein content, mineral content and heavy metal contents in the glutamate thalli protein of sampled from four factories were determined. Furthermore, apparent metabolitic energy (AME) and amino acid digestibility of glutamate thalli protein were determined by metabolitic trial. The results of this paper are summarized as follows:The first, this paper investigated the character of monosodium glutamate waste water, output of glutamate thalli protein, the technology and methods of collecting glutamate thalli protein in China. There are four kinds of methods of extracting glutamate thalli protein: high-speed centrifuge, ultrafiltration, heat and precipitate, flocculate and precipitate or air floatation, but the fourth is main method used in factories in China. The choice of flocculant and reactive terms control are very important. Exploiting and utilizing glutamate thalli protein can not only alleviate the shortage phenomenon of protein feedstuffs in our country, but also lighten the pollution of the environment, have great economic benefits and environmental benefit.The second, the routine nutrients, true protein, amino acid, minerals, pH value and heavy metal contents in the glutamate thalli protein were determined. The results indicated the glutamate thalli proteinhas very high crude protein content, even surpassing that of fish meal, but its true protein content, amino acid content are relative low, amino acid constitute of glutamate thalli protein is imbalance. Among all the amino acids, the content of Glutamic acid is the highest, however, that of Cystine is the lowest. There are plentiful minerals in glutamate thalli protein, but calcium and phosphoruscontents are low, morever the ratio of calcium and phosphorus is not proper. The heavy metal elements contents are relative low and safe for animals except mercury.The third, 15 nongdahe adult roosters which its` average weight is 2658g were divided into 5 groups at random, 3 roosters every group. The trial use 5 ×5 latin design to determine the AME of glutamate thalli protein with forcefeeding-fasting method. The results revealed that the AME of glutamate thalli protein is low as a whole, the AME of Ⅳ(2887.5Cal/g) is highest, and that of l is the lowest(1443.3Cal/g). There is significant difference between them.The fourth, 20 nongdahe cecectomized adult roosters were divided 5 groups to determine the amino acid digestibility of glutamate thalli protein, 4 roosters every group. The results indicated that the apparent amino acid digestibility and true amino acid digestibility of the four kinds of glutamate thalli protein is relative low. Almost all the apparent digestibility of glutamate thalli protein is below 80%. Most of essential amino acid true digestibility of Ⅳ is above 80%, while others is below 80%. Expect the Cys and Arg true digestibility of Ⅳ is equivalent to fish meal, others are far below that of fish meal and soybean meal. There is significant difference between them (p<0.05) or (p<0.01). There are significant difference between apparent digestibility and true digestibility for almost all the amino acids, expect Met, Trp ofⅠ, Met, Ala, Gly of Ⅲ and Ala of Ⅱ.