Study On Intrauterine Insemination Of Sheep

Pregnancy rate of artificial insemination of sheep is low, especially for frozen semen. The method of intrauterine insemination(i.e. laparoscopic intrauterine insemination )can improve artificial insemination efficiency. In this study, the mode, time, site, effective spermatozoa of intrauterine insemination were ascertained, and insemination technique has been ameliorated so as to be generalized during the production. In addition, Estrus induction of anoestrus season and estrus synchronization of breeding season also have been compared, and their insemination effect to superovulation of donor. The results validate the merits and maneuverability of laparoscopic intrauterine insemination. The results show that:1.Under the existing experiment condition, the method of intrauterine insemination means that uterine cornu was fetched out with uterine forceps to eject semen outside peritoneal cavity, infuse semen into uterine cornu, Insemination operation's apparatus is simple , means is easy, pregnancy rate is the highest, tends to popularize and application.2. Intrauterine insemination time are different. Ewe of naturally cycling is inseminated after 24 hours of oestrus; Ewe of Estrus Induction is after 54 hours of device removal; Ewe of estrus synchronization is after 60 hours of device removal. Pregnancy rate of oestrus ewe is the best if it insemination effect at 2-3 hours before finishing oestrus, viz. ovulating.3.Applying middle section insemination of the bilateral uterine cornus, insemination effect is better than unilateral uterine cornus4.If insemination effective spermatozoa are 40 millions, insemination effect is the best. Besides, and insemination effective spermatozoa is tween 20 millions to 40 millions, insemination effective spermatozoa and pregnancy rate are linear relation.5. The upmost insemination pregnancy rate of frozen semen for the ewe of naturally oestrus is 57.14%(4/7), the ewe of estrus induction is 62.50%(20/32), the ewe of estrus synchronization is 55.56% (5/9) . The application experiment of insemination confirmed that intrauterine insemination can improve pregnancy rate , twinning rate of insemination ewe and impregnation rate of embryo for superovulation donative ewe.Postgraduate: Liu Yufeng Major: Animal genetic breeding and reproductionSupervisor: Prof. Li Wu...