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Studies Of Blood Protein Polymorphism In Xinjiang Wapiti

Posted on:2003-08-07Degree:MasterType:Thesis
Country:ChinaCandidate:X M XingFull Text:PDF
GTID:2133360065960177Subject:Conservation and Utilization of Wild Fauna and Flora
Abstract/Summary:PDF Full Text Request
Thirteen blood protein Loci of three deer breeds in Xinjiang (Tianshan Wapiti, Aertai Wapiti and Talimu Wapiti), which included Erythocyte Haemoglbin(Hb), Erythocyte Esterase(Es), Serum Prealbumin-2(Pr2), Albumin(Alb), protease inhibitor-1 (Pi 1), protease inhibitor-2(Pi2), Vitamin D binding protein(Gc), slow a -glycoprotein(Sag), pastalbumin(Pa), pretransferrin(Prtl),Pretransferrin(Prt2), Transferrin(Tf), posttransferrin(Ptf), were detected by methods of vertical polyyacrlamid gel electrophoresis .The results showed that Hb, Prtl, Pa, Prt2, Sag, Ptf were controlled by two codominant alleles, and Tf was controlled by three ones. Only one Phenotype in plasma was detected at Alb, Gc, Sag, Pr2 Loci, and 2, 2, 1 phenotypes at Ptf Locus, and 2 phenotypes at Hb and prtl, and 3, 3, 2 phenotypes at Tf Locus. Pa Locus took on 3 phenotypes and Pra2 Locus was 2, 3, 3 phenotypes, bus no detectation was done at Es^ Pil and Pi2 Loci. Genetic analysis for crossing deer showed that the genetype of crossing deer was Gene-recombination of their parents.It also showed that all breeds for different Loci of phenotypes lie in Hardly-Weinberg equilibrium state except Hb Locus in Tianshan and Hb> Prt2> Pa> Loci in Talimu,the average heterozygosity of polymorphism Loci for Xinjiang Wapiti was 0.2675 ^ 0.2507 and 0.1545 respectively.The cluster analysis showed that the genetic distance between Tianshan and Aertai is 0.0489, and the next is Aertai and Talimu(D=0.0614), and the relationship between Tianshan and Talimu is the farest (D=0.1601), this indicated that it could be available for Antler deer distinguished by the methods of protein polymorphism and discussed correlation between different breeds.Regression analysis between different saw(l~6) and antler output concluded that the regression formula is: Y1=0.9833X]+2.3134 and theregression equation for 1-5 saw deer and regeneration antler output is:Y2=0.2840X2+0.3947.General linear model was founded for the selection of biochemical genetic marker and by x2 fitness test, it was estimated that there is significant difference(P<0.05) for Hb and Prt2 Loci and the reproduction survival rate and also for the Hb Locus and the main part of Antler.The effect value analysis showed that it is up to significant difference(P<0.05) for AA type of Prtl Locus and the weight of birth. AA type of Hb Locus and reproduction survival rate and also for AA type of Prt2 Locus and the dropping pedicle date; These results indicated that there was genetic correlation for some genetypes at Loci of protein polymorphism and the three properties(economic characters including reproduction, production and Antler output), therefore, we can use the AA type in Hb Locus, the AA type in Prt2 and the AA type in prtl as the deer's marker to make the breeding of Talimu.
Keywords/Search Tags:Talimu Wapiti, Tianshan Wapiti, Aertai Wapiti, blood protein polymorphism, ecenomic characters
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