| Two experiments were undertaken with attempt to produce the chicken meat rich in polyunsaturated fatty acids and low in cholesterol content. Experiment I was conducted to study the effects of dietary different oil sources on the fatty acids composition and cholesterol content in chicken meat; Experiment II was conducted to evaluate the effect of different levels of linseed oil and copper on the fatty acids composition and cholesterol content in chicken meat.In experiment 1, ninety 83-d-old yellow female chicks were randomly divided into five treatments (two replicates pens per treatment, 9 birds per replicate) to study the effect of dietary different oil sources on the fatty acid composition and cholesterol content in the liver, muscles of breast and thigh, and serum parameters of the chicks. Diets with 3% palm oil (PO, CT) rich in saturated fatty acid, 3% or 5% Linseed oil (LO) rich in alpha-linolenic acid (C18:3 n-3,ALA), 5% fish oil (FO) rich in eicosapentaenoic (C20:5 n-3) and docosahexaenoic acid (C22:6 n-3,DHA), 5% huoma oil (HO) rich in linoleic acid (C18:2n-6,LA) were fed respectively. On days 21 ,28 ,35 of experiment, fatty acid composition and cholesterol content in liver and muscles of breast and thigh, and serum parameters were determined and other performance parameters were recorded. The result showed the fatty acids profiles of lipid extracted from the tissues of experimental chicks completely reflected those of the diets. PO in diet resulted in higher n-6/n-3 and cholesterol content in liver and muscles of breast and thigh, and higherLDL(low density lipoprotein) and TG (total triglyceride) in serum than other treatments. Serum HDL (high density lipoprotein) and TC (total cholesterol) had no difference between each treatments (p>0.05). Compared with contorl, LO (3% or 5%) increased the amount of polyunsaturated fatty acid (PUFA), mainly in the form of ALA and LA. The n-3 long-chain PUFA content of 3% or 5% LO treatment was slightly higher than in 3% PO treatment. The amount of PUFA increased when the diet containing 5% FO was fed, mainly in the form of long-chain n-3 FA (EPA , DPA and DHA). Huoma oil increased the amount of PUFA, mainly in the form of LA, levels of total n-6 were higher than other treatments. Linoleic acid and linolenic acid was preferentially deposited in thigh muscles and long chain polyunsaturated fatty acids (AA,DHA) in breast muscles and stearic acid and oleic acid in liver. With longer feeding time of diets containing LO and FO, the ratio of n-6/n-3 were decreased, but that of feeding 5% HO was increased.In experiment 2, a 3X3 factorial arrangement of treatments was used: 3 levels linseed oil (0%,3%,5%) and 3 levels (Oppm,125ppm,250ppm) copper as copper sulphate pentahydrate. One hundred sixty-two 83-d-old yellow female chicks were randomly divided into 9 dietary treatments: 0%LO +0ppm Copper(0/0, CT), 0%LO +125ppm Copper (0/125),0%LO+250ppm Copper(0/250), 3%LO +0ppm Copper (LO3/0), 3%LO+125ppm Copper (LO3/125), 3%LO +250ppm Copper (LO3/250), 5%LO +0ppm Copper (LO5/0), 5%LO+125ppm Copper (LO5/125), 5%LO +250ppm Copper (LO5/250), to study the effect of dietary LO and copper on the composition of fatty acid and cholesterol contents in the liver, and breast and thigh samples and serum parameters in chicks. Every treatment was replicated 2 times with 9 birds per replicate. On days 21 ,28 ,35 of experiment, fatty acid composition and cholesterol content in liver and breast and thigh samples, and serum parameters were determined and other performance parameters were recorded. With the LO level increasing, the content of PUFA in liver and breast and thigh samples was increased, and the ratio of n-6/n-3 fatty acidwas decreased. High-level copper supplementation decreased the long-chain PUFA and decreased cholesterol content in breast and thigh and liver lipids. The cholesterol content of chick tissue between 5%LO and 3%LO treatment was not significant differences (P>0.05). But dietary high level copper could decrease the cholesterol content (P<0.05) and decreased s... |
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