Studies On The Rapid Identify Technology For Turfgrass Seed Cultivar

The identification of turfgrass seed cultivar truthfulness is an important index to assess the quality of grass seed, and important premise to assure the lawn produce safely and circulate smoothly. The traditional identify method for using plant morphologic character has been proved is not enough. The research on new and effective technology of breeding identify has been the chiefly task of turfgrasses seeding production. So in this paper, the isoelectric focusing electrophoresis technology has been used to identify several kinds of the cold-season turf grasses breeding truthfulness in west -north region, and the electrophoresis isozyme bands analysis software (ImageMaster ID) has been used to analysis the peroxidase isozyme bands of the turfgrasses. The reliability of this technology to identify turfgrasses cultivar has been proved in different way. The result as following:1 -. The key of using the isoelectric focusing electrophoresis technology to identify truthfulness, is to choose different pH value ampholyte for different plant species. Lots of experiments have proved that, the peroxidase isozyme bands of several kinds of the turfgrasses are more clear and more easily to identify at pH4-6 value gel.2. At the different growth period, the composition and content of isozyme have changed. The age of the turfgrasses cultivar effects the electrophoresis bands clear degree, so effects the identify results. The experiment proves that, The best experiment materials of Lolicem perenne, Festuca rubra, Festuca arundinacea Schreb and Trifolium repens are the seedlings of 6-8 days after germination. The best experiment materials of Poa pratensis is the seedlings of 10-12 days after germinate.3. During the experiment we discover that, under all added 25 ul water extract condition, the enzyme extract liquids concentrations of these surfgrasses cultivars (the roots been cutted) are different, so affect the results. The experiment shows that, under the condition of added 20wl water to extract the enzyme, the results of Poa pratensis and Trifolium repens are better, the others are less.4. Using software analysis, the examine results have been quality and quantity assessed. It fully indicates the relativity and diversity among different cultivars. Through clustering analysis, the far and near degrees of the heredity relations between breeds have been measured, and the experiment result has been proved basically accord with the results of plant taxology and indoor planting. The results show that the isoelectric focusing electrophoresis technology is effective measure technology to identify turfgrasses cultivar.