Fingerprinting Analysis On The Homozygous 13/17 Robertsonian Translocation Pig Population By RAPD

The homozygous pig population for 13/17 Robertsonian translocation with 2n=36, XY or XX rob (13;17) is a new genetic type in the domestic pig. which was obtained by means of mating the male and female heterozygous pig of 13/17 Robertsonian translocation. Random amplified polymorphic DNA (RAPD) techniques were used to investigate the genetic structures of the homozygous 13/17 Robertsonian translocation pig population, the heterozygous 13/17 Robertsonian translocation pig population and the normal pig population. Three DNA pools of the populations and 14 individual DNA samples were used. Sixty random primers were screened, of which, amplified result from 22 primers were polymorphism while the other 8 were monomorphism. The fragment length of products from 30 primers in different samples were between 200 and 4000 bp and the band numbers were between 6 and 14, which were distinctly and easy to be distinguished. The fragment shared (F) and the genetic distance (D) was calculated based on it. The genetic distance indices matrixes indicated that it was between 0.012 and 0.033 in 3 populations and between 0.010 and 0.039 in 14 individuals, the genetic distance indices between and within the population of the 13/17 Robertsonian translocation were low, The polygenetic trees ~vere drawn, which showed the genetic relationship among 3 populations and 14 individuals objectively and explicitly. The genetic diversity index (Ho) illustrated as that: Ho in the normal pig population was high (0.078~-0.198). while it was low in the heterozygous for 13/17 Robertsonian translocation pig population (0-.-0.176) and lower in the homozygous for 13/17 Robertsonian translocation pig population (0~0.174). The genetic variation among the populations is greater than that within the populations. The genetic diversity index (Ho) of the homozygous and heterozygous for 13/17 Robertsonian pig translocations is significantly lower than that of the normal pig population (p<0.0 5), while the difference between the homozygous pig population and the heterozygous pig population is not significant (p>0.O5). It was concluded that the similarity conclusion on heredity and variation in populations by the genetic distance indices and the genetic diversity indices, and it was convenient and. feasible and effective in 47 13/17 ~4*~#~ IL*~*i~.~. DNA j~~~4Jj closely genetic related. In the analysis b~?RAPD, a normal pigs specific fragment (265bp) was found, which could not he amplified in the other Robertsonia translocation populations; it indicated that it might be the lost fragment during the formation of 13/17 Robcrtsonian translocation. The specific amplified fragment has been cloned and sequenced with pMDI 8桾 vector, it would be the foundation of further molecular genetic marker research.