Study On The Changes Of Endogenous Hormones And Proteins During The Cuttings Rooting Of Larix Olgensis And Its Hybrid

The softwood cuttings propagation for Larix olgensis in 5 year old and its hybrid(Lahx olgensisX Larix Ieptolepis) in 7 year old has been done. The materials were treated with exogensis hormones of IBA and NAA in different concentration for different time. Several endogensis hormones included Auxin IAA, abscisic acid ABA, cytokinin ZR and gibberelin4 GA4 within the cuttings and their mother trees were analyzed by using the enzyme-linked irnmunosorbent assays (ELISA) technique, and soluble proteins also were tested by the sodium dodecyl sulphate polyacramide gel electrophresis (SOS-PAGE). The object was to analyze the relationship among the endogensis hormones, exdogensis hormones, soluble proteins and cutting woods rooting and the relationship each other. The results showed as follows. (1) There is significant variation in the root rate among different cuttings treated in BA and NAA, and the two best treatments are the BA 200ppm in 8 hours and BA 600ppm in 4 hours, while the treatments of NAA doesn抰 improve the cuttings rooting. (2) According to the change of endogensis hormones in mother trees and the woodened condition of the cuttings, the best time of the softwood cutting propagation for Iarix olgensis is determined in early July in the middle and south area of Heilongjiang province. (3) it also showed that the cuttings from No.3 clone get the highest rooting ability from three clones, results analyzing the endogensis hormones in the three different clones show that the content of ABA in the hightest root rate clone No.3 is the lowest than other two clones, while its ABA concentration was the lowest during early stage of the cuttings, and visa versa for the other two clones. That means it has some constraint effect for ABA constraining to the cuttings rooting. ZR and GA4 concentration in No.3 clone are higher than that of that of the other two clones. It can been seen that ZR concentration in the cuttings keeps in higher level during its all rooting time, and the higher in GA4concentration in the cuttings is just during the differentiation of root primordiu. There is no clear change for IAA concentration in the cuttings from all clones during cuttings rooting. (4) During the cutting, rooting the soluble proteins of 47kd, 39kd, 33kd, 28kd, 24kd are related to the rooting. The soluble proteins in 39kd and 24kd control the callus growing and adventitious roots differentiating, and they disappear when the cuttings star to get adventitious roots . The soluble proteins in 28kd and 33kd appear during the callus and root developing. It seems that that the soluble protein in 47kd in the cuttings has some disadvantage to the callus differentiation of the cuttings rooting.