| In this study, one pair of primers ( gpl/gp2 )from gpSO gene and other two pairs of primers (gEl/gE2,gE3/gE4)from gE gene, have been synthetized based on the gene sequences of PRV gpSO and gE genes.The primers, gp 1 /gp2 amplified a fragment with desired size of 217 bp and the other two sizes were 985 bp & 934 bp respectively. PCR technique was applied for the detection of PRV in localized areas of Guangxi province.The results showed that 31 of 53 samples were positive, the positive samples detected by gpl/gp2 primers were also positive detected of HCV by PCR. Using two pairs of primers of gE gene, all of the amplifications of standard PRV MinA strain were positive, however, the primers can not amplify any fragment of PRV vaccine NIA3-783 strain. Rabbits showed typical syndrome of PR when they were inoculated with positive samples, the samples can also caused typically cytopathic effect (CPE) on RK and PK- 15 celles. In the detection of different sites of porcine infected of PRV ,the highest detection rate was in the brain, the secondary was tonsils, liver, spleen and kidney, One of two samples that picked from healthy pigs was positive by PCR. Results of this study proved that PR is still an epidemic disease in Guangxi currently. It is worth to set eyes on the problem of PRV latency infection and infect with other virus. This experiment lay a good foundation for studying PR molecular epidemiology in Guangxi and the method to differentiate PRV field strain from attenuated vaccine. |
