Study On The Technical System Of Tissue Culture In Euphorbia Pulcherrima

Euphorbia pulcherrima is one of the most important potted flower, a few of researches about tissue culture of Euphorbia pulcherrima have been reported in the world. In view of keeping the hereditary property, a rapid propagation method of tissue culture in Euphoria pulcherrima was established, in which Orthogonal design, randomizing scheme and multivariate statistical analysis were employed in the experimentation. The results were as follows: The optimal explants were tender leaves (with partial veinlet), shoot tips of end buds or lateral buds from Euphorbia pulcherrima in 5 month. Explants were sterilized in 0.1% mercuric chloride for 7-8 minutes after washed in tap water for 2 hours and sterilization of 70% alcohol for 8-9 secends, Bloting the cultured material could prevent browning effectively when inoculating it on callus-inducing culture medium. Callus-inducing culture medium was the MS medium with 0. lmg/L BA. l.Omg/L 2,4-D and 3%sugar, or with 0.1 mg/L NAN~ O.lmgIL BA~ 0.Smg/L 2,4-D and 3% sugar. The subculture medium was the MS medium with 0.Smg/L BA.~ 0.lmg/L NAA and 3% sugar. Theoretically, 38 the coefficient propagation of a shoot tip could reach 5-6 for 15-20 days, so the proliferation times of a shoot tip could reach 2.2 X i09 for a year. It was a effective method in numerous proliferatin of seedling of Euphorbia pulcherrima in short term, then the new buds were eventually transferred to the 1/2MS medium containing 1 .Omg/L IBA. 1 .Omg/L NAA and 3% sugar, the rooting rate could reach 80%, Moreover, in order to reduce production costs, paclobutrazel was utilized in tissue culture for other抯 phytoregulators, but It was not marked without sugar in rooting culture medium. All the cultures were kept under the temperature of 25? ~慍 ,illuminated for 16 hours every day, and the light intensity was 1 500-25001ux. Finally, the survival rate could reach 50% when the hardened rooted test tube plants was transplanted into the medium with 1/3 vermiculite 1/3 ruby mica and 1/3 coconut husk.