RAPD Marker For Salt Tolerance Gene(S) And The Cloning In Wheat

Genetic and RAPD (Randomly Amplified Polymorphic DNAs) analysis studies were carried out in F2 population originating from a cross of two wheat cultivars i.e. Nongda 85021 (salt sensitive, female parent) and Chadian Red (salt tolerant, male parent). For genetic analysis, chi? square test gave a good fit to 1:2:1 ratio showing thereby that one major gene was controlling the salt tolerant character. 10 each of the salt tolerant and salt sensitive plants were selected for DNA extraction to develop tolerant and sensitive gene pools respectively. From the BSA (Bulked Segregants Analysis), RAPD technique, 520 random decamer primers were used to amplify the two types of gene pools, only one primer(OPZO9) detected and was found 1 fragment of 590bp was polymorphic. Using the primer to amplify the parents, El and F2 populations, the specific fragment OPZO9?90 was a RAPD marker linked to salt tolerance of Chadian Red. By the software JOINMAP(Versionl.4), the recombination was 5. 674% and the linking distance was 6.557cM. Fragment OPZO9?90 was extracted from the agarose and mixed with pUCm vector, then transferred into JM109. After the recombined vector was extracted and digested with EcoR I and Hind III, then detected by electrophoring in agarose, the results confirmed that the OPZO9?90 has been cloned successfully. The bases sequence of the RAPD was measured and showed that the marker was OPZO9?91. This research provides a base for further on markerssistedelection for salt tolerance in wheat and possibility of gene tagging and cloning for salt tolerance.