| The monosomic addition line of sugar beet M14 (2n=18+1) was produced by interspecific cross of cultivated Beta vulgaris with wild species Beta corolliflora Zoss which is considered to have apoximic characteristics. Therefore, the sugar beet hybrid M14 has the special genetic phenomenon with different chromosome numbers. In this experiment we examinated in three consecutive years hundreds of progenies of sugar beet M14 from our laboratory and made statistical analysis on the base of chromosome numbers observed with the help of optical microscope. The findings showed that the average number of progenies with 19 chromosomes of sugar beet M14 is 36.3%. We microdissected in vitro 19 chromosomes of sugar beet M14, then applied PCR technique with four pairs of specific primer DNA sequences, which come from Beta corolliflora Zoss chromosomes to check 19 chromosomes. Our results showed that all of the four pairs of specific primer DNA sequences were no more target wild chromosome-specific. This result suggested that alien chromosome fragments has integrated into sugar beet M14 and made a nature recombination. In order to understand the mechanism of the special genetic phenomenon of sugar beet M14 and do basic work for exploring and utilizing genetic resources of sugar beet, we selected and microcloned one of the 19 chromosomes, and finally isolated a new gene which is called m14-2c1. Based on biological information of its structure and function, we found that this gene has some homologous characteristics with the ARID family. We report here for the first time a new gene, which needs to be further study for its role in the specific genetic mechanism.
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