Study On Key Technology Of Standardized Production

Houttuyniae herba from Fresh grass or dry all parts on the ground of medicinal and edible plants saururaceae Houttuynia cordata genus Houttuynia cordata Thunb.. Its function is to remove toxic heat and anti abscess and diuresis to drench, medicine for the treatment of pulmonary abscess.As one of the most development potential resources in medicinal and edible plants, Houttuyniae herba has the very broad development use value. But due to planting patterns and long-term asexual reproduction, led to the degeneration of variety. The medicinal materials quality and the yield difference is very big, and the quality of existing legal standard evaluation method is not perfect, so that the quality control of houttuyniae herba more difficult.This subject for the first time the GC/MS method for the simultaneous determination of four constituents (4-Terpineol, Alpha terpineol, Borneol acetate, Undecanone) in the essential oil content, to evaluate the quality of herba houttuyniae medicine.This paper mainly study the key technology of the gap standardization production, as to provide theoretical basis for building standardization production technology, so as to ensure the quality of medicinal materials from the source.Resource distribution:The literature suggests that houttuyniae herba are mainly distributed in the southern provinces in our country, and found that the areas are distributed in Sichuan. In combination with field investigation, can undertake its GAP base construction in shifang, guanghan.Germplasm resources:By comparing agronomic characters of49germplasm resources, which are from10provinces,The results show that All the germplasm pharmacopoeia is variety, and found no specific populations; Based on the yield and composition of volatile oil content, four kinds of weighted comprehensive score, The Score results show that the germplasm resources of09,10,11,21,30,39varieties can be used as its good varieties of breeding materials, including germplasm resources09,30mark for the best, in more than90points.Breeding material:Through to the10batches of seed germination experiment, the results showed that the seed germination rate up to91.33%,60%of the seed germination rate of more than70%. Based on940strains of the plant stems K-class center clustering method, the data in internode diameter, stem length, internode length for classification indexes, will be conducted in the stem is divided primary, secondary, tertiary, and substandard level.Cultivation techniques:According to its different growth condition, can be divided into medicinal (month B to H) with edible (month I to K) two stages. By investigating the seeding method and rate,Stem dosage, fertilizer rate, recovery of fixed number of year, harvest time. Houttuyniae Herba standardized production base appropriate chooses underground part as asexual reproduction material, seeds in the middle of B to early D, with a kind of Y Kg per acre. Basal to compound fertilizer Q Kg per acrea and phosphate fertilizer T Kg per acre.Seedling stage,Growth period, Sealing the date according to three times, each use of the urea M Kg per acre. Medicinal houttuynia herba can harvest two years in a row in G month to the middle of H month.After the harvest of medicinal, Adopt the way of coated in consumption of its cultivation, about90d with the harvest.Quality standard:Through the measurement and analysis heavy metals, pesticide residues, nutrients of3batch houttuynia herba of edible, And set the standard of heavy metals limits, pesticide residue limits and the content of nutritional composition (moisture, Vc, crude protein, crude fiber) for edible houttuynia herba of the base production.Through the thin layer of10batches of Houttuynia cordata medicinal material, examination, extract, content determination of4kinds of volatile and oil, Supplement and perfect the quality standard, It Improved the limit of moisture and the content of extract, and Increased the pharmacopoeia standard contains no limited, heavy metal limited, the total ash content of pesticide residue limits limited, naphtha, aflatoxin content and four components (4-Terpineol;Alpha terpineol;Borneol acetate;Undecanone) content determination standard. This will be to ensure the stable quality of herba houttuyniae medicine, controllable, and provides theory basis for the clinical use of effective, to reflect objectively and evaluate its quality.