| Recently, fast and high-precision biomedical detection has been broadly applied in basic biology research, clinic inspection and other areas. However, current inspection instruments are too huge and expensive for promotion. Surface plasmon resonance (SPR) biosensor is one of the latest detection method based on physical optics. Its characteristics include but not limited to high sensitivity, fast inspection, low sample consumption, no label and zero damage, so it has been widely used in surface inspection, biomedical (biochemical) analysis, drug screening, environment monitoring, and so on. So far, as an important technology to study interactions between biological macromolecules, it has obtained wide attention from domestic and international scientists. Based on the study of many relative research achievements and combined SPR sensing technique and microfluidic technology, a portable, high-precision biomedical detection system has been designed to meet some practice requirements, such as automatic, fast, small size and high precision.In this thesis, the principle, application area, current status and future development of SPR technology is introduced at first. Then detail design of the system is discussed, including inspection system and a microfluidic chip based on new technique. The impact of each parameter on the SPR system design is simulated by using Matlab software, which gives a theoretical base for the choice of suitable parts in device design, and a new detection system, combing optics, mechanism and electronic technology, has been designed. According to the Kretshmann theory, cross-arm mechanism structure and linear actuator are used to realize precise equal-angle movement and let the image sensor accurately capture optic signal. Laser-cutting method, as a novel machining technology, is tried to fabricate the microfluidic chip. Result of preliminary experiment shows that this device is basically met the design requirements such as compact, portable, stable and highly precise.
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