Key Technologies And Applications Of Full-automatic SPR Analyzer

Environmental and food suffers the serious pollution problem today, the toxic organic pollutants widely distributed in the environment, a wide variety, low concentration, rapid, sensitive and accurate analysis of detection face great challenge. At present the main detection method with chemical method, biological method and immune analysis method, these methods are different aspects of the problems, cannot meet the growing demand for the analysis of the mass rapid detection, such as instrument is expensive, complex operation, time consuming, etc.. Biosensing technology, especially the bio-chip based, of which the surface plasmon resonance (SPR) sensing technology as the chief representative, in recent years has become an international hot spot in the field, and got rapid development. And successfully applied to environmental and food safety testing, clinical diagnosis, drug screening, and many other fields.By studying the key technologies of SPR analyzer, independent intellectual property rights of full-automatic SPR biochemical analyzer was developed in this thesis, and also carry out several rapid detection of trace organic pollutants, good results were achieved.Several relevant peripheral technologies and key technologies of SPR analyzer were disassembled for research and design, and relevant application experiments were carried out, the main work is as follows:1. Two kinds of microfluidic SIA system were designed, miniature type and full-automatic type, respectively. Air separation methods was used to protect the sample from buffer. The problem of the air segment flow system precise injection under different pressure was solved. This system running stable, the effect of isolation and separation of samples-buffer is good.2. To the features of SPR sensor signal temperature sensitivity, temperature compensation method is studied. Research and experimental results show that temperature variation compensation is not ideal, and combined with the velocity of temperature variation compensation is improved, but still difficult to practical application, thus designed based on TEC and the PID temperature control system.3. Proposed improvement program to optimize multi-channel SPR sensor. Use 760nm wavelength LED light source and add narrowband filter; Use 16 bit AD CCD instead of 8 bit planar CMOS; Better resolution and dynamic range will effectively enhance on existing sensor.4. Two integrated microfluidic chip were designed, interconnection channels type and controllable channel type, respectively. External control circuit is developed to meet the SPR biosensor chip surface detection of liquid flow requirements.5. A fast SPR resonance angle algorithm named fixed boundary centroid method was studied and proposed. By performance comparison of fixed baseline centroid and dynamic baseline centroid using experimental data, the results show that the method has the best comprehensive performance.6. Research on a real-time sensorgram noise smoothing algorithm. Using the algorithm to processing the experimental data for noise reduction, analyze and evaluation the algorithm performance. For the typical SPR sensorgram, the noise level can be reduced to 3.5% of the original, and with little signal distortion and data delay, improved the instrument detection limit.7. Applying the self-development miniature and full-automatic SPR analyzer conducted sulfamethoxazole, bovine serum albumin, imidacloprid biosensor detection experiments, respectively, with good test results. And also compare with Biacore instrument.