Extracting Phycobiliprotein From Spirulina And Research Of Dsscs' Anode Sensitizing By Phycobiliprotein

This paper summarized the spirulina, phycobiliprotein and the structure, basic working principle and development of dye-sensitized crystalline TiO2 photovoltaic solar cell. On the bases of them, this paper investigated the spirulina cultivation, phycobiliprotein extraction and sensitizing process of phycobiliprotein on DSSC'anodes, etc.Firstly, the spirulina was cultivated by"culture medium Z"in our laboratory for increasing, then growth situation of the spirulina was periodic observed and biomass-time coordinate curve was draught. On the bases of that, a cultivating method of using wastewater and seawater as the nutrition supplier was investigated, and the most appropriate proportion of wastewater and seawater was obtained, so it provided low-cost raw material for the large-scale phycobiliprotein extraction , and purified the wastewater.Phycobiliprotein was extracted by method of turgid. Influencing of extract temperature, time and quantity of water factors on phycobiliprotein extraction was investigated by orthogonal experiment.The results showed that the most appropriate extracting temperature was 35℃, extracting time was 6 days and quantity of water was 2.868ml per mg of dry spirulina.Butyl titanate, grain alcohol, diethanolamine, polyethyleneglycol and purified water were chosen as raw material, then TiO2 film was spreaded on the electric glass by sol-gel processing, then sensitized the film by the extracted phycobiliprotein, made the dye-Sensitized TiO2 anodes. Concentration of phycobiliprotein, temperature and time of sensitization were inspected by orthogonal experiment to explore their affection on sensitizing. As the results showed, the most appropriate concentration of phycobiliprotein was 308.94μg /ml, sensitizing temperature was 15℃and time was 40min. The most influential factor was the concentration of phycobiliprotein. Dry graphite was spreaded on electric glass to made the counter electrode, then DSSC was assembled, using saturated solution, which dissolved I and KI by acetomitrile as electrolyte; and dye-sensitized TiO2 anodes, tested the DSSC with relevant anodes, explored the affection of sensitizing process made on the performance of DSSC, besides, inspected sensitizing effect of phycobiliprotein within lanthanum nitrate and gadolinium nitrate respectively, the results showed that metal ion doping can improve photovoltage of DSSC evidently.