Determination Of ·OH By HPLC And Study On System Of Ballast Water Treatment With The Rate Of 5t/h

Ballast water,considered as one of the four threatens,lead to biological invasions which destroy our ocean environment seriously as well as make a heavy loss to our country's ocean shipping corporations and economy.Today,our country already ranks treating the ballast water as an important scientific research project.And in this study the author combined with the National Key Technology R&D Program "Technique of treating the ocean shipping ballast water by hydroxyl radical",designed the experimental system which was used to simulating ballast water,and detected relative objects.·OH(hydroxyl radical,·OH)is a strong oxidability substance which is only inferior to fluorin.Because of its short existence time and low concentration,its measuration is quite difficult,and exact analyzsis and measuration method becomes rather important. The paper determined·OH through the method of high efficiency liquid chromatography,which chose salicylic-acid as catcher,and the mobile phase proportion chose 5%carbinol and 95%acetic acid-acetic acid-Na.And the author investigates the influence factors of·OH measuration.Results showed that under the acidic conditions,·OH was restrained,in turn,it gone against to formation of·OH.However, under the alkaline conditions,it was propitious to the formation of·OH,and the increase of formation amount in alkalescence condition was higher than it in acidity condition. With the increasing of reaction time,the formation amount of·OH was gradually increasing.When reaction time reached to a certainty time,the formation velocity tended gentle.The salicylic-acid was propitious to increasing the mass transfer efficiency of O₃ in the water,which influence the formation of·OH,so under the same condition,the change of concentration of substrate could affect the formation of·OH. When voltage reached to a certainty value,it appeared an optimum voltage under which was best propitious to the formation of·OH.In the experiment,the study made some conclusions:when the voltage value changed from 180V to 190V,the increasing of formation amount of·OH wasn't very distinct.And the water quality also influenced the formation of·OH.The concentration of formation amount of·OH in seawater was 5.2429μmol/L.After understanding the technological process of treating ballast water,author chose suitable materials to design the experimental system used for simulating the ballast water.Under the requirement of G8 Guideline,author chose Isochrysis galbana and Syneehoeoccus as experimental object to simulate the ballast water,and had a killing experiment at different concentrations of 10⁴cell/ml and 10³cell/ml.The lethality rate at the two different concentrations reached to 99%,and the concentration of all the live algals met the provisions of D-2.Under the alkalescence condition,changing the value of pH was propitious to decreasing the algals'killing time,in turn,increased the efficiency.Research fully demonstrated that:online treating the ballast water during transport process,provided the related basis for ship-board experiment and laid a foundation for effective green method of treating the ballast water for ships.