| As an important trend in analytical chemistry, microfluidic analysis system has become one of the most active fields of research. It aimed at the miniaturization, integration and portability of analytical instruments. In this paper, a miniaturized chemiluminescence (CL) analysis system integrating a micro-pump was established, which was characterized by simple configuration and high integration. The micro-pump was designed based on capillary and evaporation effects. Then, a multichannel CL analysis system was primarily studied.In the first chapter, the techniques of sample introduction, fluidic drive and detection for microfluidic systems were reviewed. The purpose and design of this work were presented.In the second chapter, a miniaturized CL analysis system with fluids driven by a pump based on capillary and evaporation effects was developed, which was composed of an automatic sample introduction device, the integrated micro-pump and CL detection equipment. Specified procedures of the system design and fabrication were given. Performance testing of the micro-pump showed the micro-pump could offer steady and reliable flow rate in regular experimental condition, and a precision of 2.8%(n=78) RSD was obtained in 400 min. Using luminol-H2O2-Co(â…¡) as the analysis model, the excessive reagents were added into the reservoir located at the end of the capillary, and H2O2 sample was driven into the reservoir by the micro-pump via the capillary. A precision of 1.6%(n=99) was obtained with a throughput of 80 samples/h and 30-nL sample consumption each assay.In the third chapter, a multichannel CL analysis system with fluids driven by a pump based on capillary and evaporation effects was primarily studied. The micro-pump, automatic sampling device and CL detection system for multichannel were established. This system laid a foundation for automation and high throughput of CL analysis.
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