Preparation And Characterization Of Molecularly Imprinted Polymers With Temperature - Sensitive Lysozyme

Proteomics is one of the most important research fields, which has a very close relationship with human health and social development. The research on the protein needed to develop methods of selective separation and identification of specific target protein from complex system. Molecular imprinting technology (MIT) based on specific recognition ability for the target molecule has become one of the ideal ways to solve this problem. By using lysozyme as the template molecules and bulk imprinting method, this work describes three types of lysozyme molecular imprinted polymer combined the temperature-responsive polymer with molecular imprinting technology, whose structures and properties were also studied.By using N-isopropylacrylamide as functional monomer, lysozyme molecularly imprinted/non-imprinted PNIPAAm hydrogels were synthesized in the method of radical polymerization. The results indicated that, lysozyme imprinted PNIPAAm hydrogels had a good temperature sensitivity in Tris-Hcl buffer solution, and the temperature sensitivity was not effected by template molecules. Imprinted and non-imprinted hydrogels had a certain adsorption capacity to lysozyme, but imprinted gel had a greater adsorption capacity than non-imprinted gel. The adsorption of imprinted gel was better than non-imprinted gel at low temperatures (T<LCST). Therefore, the selective identification process can be controlled by the temperature intelligently.Then, lysozyme molecularly imprinted/non-imprinted PNIPAAm/CA hydrogel films were synthesized by using sodium alginate as the main functional monomer. The results showed that imprinted and non-imprinted hydrogels film had a certain adsorption capacity to lysozyme, but imprinted gel film had a greater adsorption capacity than non-imprinted gel film. The adsorption capacity and selective adsorption increased with the decrease of film thickness. The adsorption capacity decreased with the increase of cross-linking agent concentration.On this basis, PNIPAAm/CA interpenetrating polymer network (IPN) molecularly imprinted/non-imprinted hydrogels were synthesized by using NIPAAm as the functional monomer and SA as the modifying agent. The results indicated that PNIPAAm/CA IPN molecularly imprinted/non-imprinted hydrogels had a good temperature sensitivity. The mechanical strength was significantly enhanced with the SA added.The IPN molecularly imprinted hydrogel had a strong selecting adsorption for Lys, and the selective identification process can be controlled by the temperature intelligently. Lys PNIPAAm/CA IPN molecularly imprinted hydrogel had better selective adsorption property to Lys by adding proper amount of SA and cross-linking agent. Fluorescent carbon dots can be used in selecting adsorption of Lys PNIPAAm/CA IPN molecularly imprinted hydrogel toward Lys.