| Chemiluminescence analysis (CL) detection was applied widely in biology, medicine and environment for its high sensitivity, wide linear range, rapidity and low-cost-measuring devices. As an automatic solution treatment method in non- equilibrium state, Flow Injection Analysis (FIA) coupled with chemiluminescence, has rapid analysis, good reproducibility, high sensitivity and wide linear range. It becomes more and more important in chemiluminescence analysis applications. Capillary Electrophoresis (CE) coupled with chemiluminescence has become an important analytical tool because of its powerful separation capacity and high sensitivity.In this thesis, we coupled the chemiluminescence with capillary electrophoresis (CE) technique, and discussed its application for environmental and biological samples analysis. The main contents and results are summarized as follows:Chapter one, introduced the main principles of chemiluminescence and the common CL reaetion systems, and the coupling methods of CL with flow injection and capillary electrophoresis. In the end, pointed out the purpose and meaning of this dissertation.Chapter two, this chapter include two sections. Sections one, the interaction of perfluorodecanoic acid (PFDA) with bovine serum albumin (BSA) was investigated using spectroscopic methods. The experimental results showed that PFDA quench the fluorescence of BSA with a static mechanism, in which hydrogen-bond and Van Der Waals force play a major role. The apparent binding constant/number of binding sites for PFDA-BSA were calculated to be 2.9471×107 L·mol-1/1.061, respectively. Sections two, in alkaline medium, perfluoroheptanoic acid (PFHA) and perfluorodecanoic acid (PFDA) could significantly enhance the chemiluminesence (CL) intensity to the reaction of luminol-hydrogen peroxide system. Based on this phenomenon, a flow injection CL method for the determination of PFHA and PFDA have been established. The optimized experimental conditions were evaluated, under the optimum conditions,calibration curve over the range of 0.02-5.0 mg·L-1 for PFHA, 0.05-7.0 mg·L-1 g·mL-1 for PFDA were obtained. The detection limit of this method were 5.0×10-3 mg·L-1 for PFHA, 9.0×10-9 mg·L-1 for PFDA, respectively. The proposed method was applied to the determination of PFHA and PFDA in spiked urine samples.Chapter three, a rapid capillary electrophoresis method was established for detecting water-soluble vitamins B1 and B6. Effects of buffer solution and voltage on the separation of vitamins were studied. VB1 and VB6 could be separated on a 50 cm×75μm capillary column with 20 mmol·L-1 borate buffer solution (pH 9.0), at temperature 25℃and electrophoresis voltage 15 kV, within 12 min, and the satisfied results were obtained with different characteristics including linearity, precision and stability.Chapter four, a quick and sensitive method for the detection of rutin in alkaline medium has been developed by capillary electrophoresis-chemiluminescence based on decreasing effect of rutin to luminal-potassium ferricyanide system. Under the optimum conditions, the CL signal was proportional to the concentration of rutin in the range of 1.0×10-7 g·mL-1 - 1.0×10-4 g·mL-1, and the detection limit (S/N=3) was 5.1×10-8 g·mL-1. For the determination of 5μg·mL-1 rutin solution, the RSD is 2.77﹪(n=5). The proposed method was applied to the determination of rutin in practical samples. |
PDF Full Text Request