High Value Research Of The Aspergillus Niger Biomass Residue From Sodium Gluconate Plant

The nutritive elements, digestible energy and chemical composition of the waste Aspergillus niger biomass from Sodium gluconate plant were analyzed a-nd evaluated, its feeding value and extracting high degree of deacetylation chit-osan by ultrasonic wave-extraction was reliminary studyed. This conslusion pro-vide scientific theoretical basis to comprehensive development and utilization of the Aspergillus niger. In addition, the hydrolysis of caulerpin also were prelim-inary studied.The results of nutrition analysis showed that the cell contains crude protein 10.30%, crude fat 3.89%, crude fiber 14.46%, crude ash 21.00%, total sugar 3 2.56%, also rich in Ca, P, Mg, Fe, K and other mineral elements. The feeding value stduy show that the total energy of the biomass is 10.91MJ/kg. The ttu-e availability of CF, EE, CF of broiler were as follows respectively:97.62%,88.97%,66.74%, apparent metabolizable energy and true metabolizable energy were6.43MJ/kg and 8.47MJ/kg respectively. The true availability of CF, EE, C F of duck were as follows respectively:100.00%,76.22%,58.85%, apparent metabolizable energy and true metabolizable energy were 5.63MJ/kg and 7.12M J/kg respectively..The residue applied in chicken feed, prepared 0-3weeks,3-6 weeks and more than six weeks of feed.Comparing the before and after adding aspergillus residue of feed costs, plus aspergillus feed costs were 3.11,3.07,3. 00 yuan/kg and with no aspergillus slag feed costs were 3.23,3.18,3.09yuan/kg. Its cost reduced 3.83%,3.44%,3.33% respectively.The ultrasonic assisted extracting high degree of deacetylation chitosan were studied. Single factor experiments and orthogonal experiment were investigated. The results showed that:ultrasonic extraction time is a key element of chitosan, the optimum conditions for the ultrasonic time was 2.5h,30% sodium hydrox-ide concentration, ultrasonic power is 250W, in this condition the deacetylation and extraction rate reached 93.86%.,9.32%.The ergosterol and protein were extr-acted during the process and comparing with the hot alkali method, The ultras onic assisted could shortened acetylation time, make reaction at room temperat-ure,the degree of deacetylation and the extraction rate of ergosterol were incr-eased by 4.57%,0.31%.Preliminary study the hydrolysis of caulerpin, the result show that:it didn't occur in acid hydrolysis, but it prone to double-hydrolysis reaction under alkal-ine conditions. In this condition of 0.2M NaOH methanol, 60℃,0.5h, we re-ceived a mixture of single and double hydrolysis, but separation and purificati-on of the two hydrolysis products were difficult. To obtain the single hydrolyz-ate further studyed impact of Pig Liver Esterase to this hydrolysis reaction, the re-sults for the reaction of 5h at room temperature only received a single hy-drolyzate product, the reaction of two-24h received double hydrolyzate.