Solid Fermentation Production Of CoenzymeQ₁₀ With Aspergillus Niger

CoenzymeQ₁₀ (CoQ₁₀) is a physiologicically important compound. It plays a crucial role in life as an electron shuttle in mitochodrual respiriratory chain. It was widely used in clinic medicine, health food and cosmetics industry because of its excellent physiology functions, such as antioxidant and cell proliferation. Solid fermentation production of CoQ₁₀ with Aspergillus niger (Asp. niger) was studied in this paper. The research conclusions were as follows:The solid fermentation of Asp. niger was studied and the optimum culture medium was obtained as follows: m(wheat bran) : m(bean bran) is 75:25, water rat of culture medium is 1 mL/g, and the original pH is about 7.0, MgSO₄ · 7H₂O, FeSO₄ · 7H₂O and MnSO₄ · 7H₂O are 3, 0.3, 0.25 mg/(g dry culture medium) respectively. The culture temperature is 30 ℃ and culture time is 66 hours. Under these conditions, the CoQ₁₀ was improved 23.1 %, reaching 0.533 mg/g dry culture medium.The optimum saponification extracting conditions of CoQ₁₀ from Asp. niger with the aid of ultrasonic was studied with the orthogonal test design. The results showed that the added pyrogallol saponified temperature and ultrasonic time affectted the saponifica-tion extracting greatly. Under the concentration of 75 % ethanol, optimum extracting condition were as the following pyr-ogallol is 8 % culture, saponified temperature is 40 ℃, ultrasonic time is 45min, the concentration of alkaliethanol was 0.05 g/mL, power is 280 W. Under the optimized condition, the extraction mass fraction of CoQ₁₀ is 0.433 mg/g dry culture medium, superior to the traditional method 69.1 %.Polyamide column chromatograph was applied for the isolation and purification primary studies of CoQ₁₀. Qualitative analysis was made by the method of TLC. The purity of CoQ₁₀ was determined by HPLC. With the different concentration of ethanol grads desorbing, CoQ₁₀ can be obtained in85 % ethanol and the purity is 91.8 %. Qualitative and identification was analysed by Thin-layer Chromatograph (TLC) and HPLC. The result showed that Asp. niger produces CoQ₁₀. The quantitative analysis metherd of CoQ₁₀ was studied by TLC separated with Ultraviolet Spectrameter (UV). The results showed TLC-UV is a fast and accurate determination for CoQ₁₀, the average recovery 90.49 % and the RSD 1.95 %.