| In the decades, people pay more attention on the quality and safety of food. At the beginning of the paper, analytic research progress about melamine, carbamate insecticides and environmental estrogens in food at home and abroad was reviewed, including the methods of analytic detection. In chapter 2, high performance liquid chromatography was applied to determine melamine in duck meat. The impacts of pH, percentage of acetonitrile and ion-pair reagent species on analysis result were investigated. The linear range was 0.25-40 mg/L with the correlation coefficient of 0.9999. The detection limit was 0.056 mg/kg. The average recovery ranged from 81.9%to 83.5%. Relative standard deviations (RSDs) were 1.8%,2.5%. In chapter 3, matrix solid-phase dispersion technology was used for sample pretreatment. Carbamate insecticides and phenolic compounds were determined by HPLC-UV. The kinds of dispersants, the ratio of weight between dispersant and sample, the kinds of eluent and volume of the eluent were investigated. The linear range of tsumacide, carbaryl, irimicarb and 1-naphthol were between 0.02-20 mg/L with the correlation coefficient of 0.9992-0.9998. The range of the detection limit was 0.02-0.03 mg/kg. The average recovery ranged from 97.6%to 105.3%. RSDs were 2.3%-2.7%. Phenol, p-cresol and o-cresol in honey the linear ranges were 0.04-16.3 mg/L with the correlation coefficient of 0.9996-0.9998. The detection limits ranges were 0.05-0.06 mg/kg. The average recovery for the three phenolic compounds ranged from 86.9%to 90.9%. RSD was 2.3%,2.3%,2.5%respectively. In chapter 4, high performance liquid chromatography was used for determination of estradiol, estrone and diethylstilbestrol in soy milk powder. The fat was removed by petroleum ether. Acetonitrile was used as extractant. Diamonsil C18 column was used. Mobil phase was acetonitrile-water (the ratio of volume was 55:45). Detector was set up 230 nm. The linear range was 0.02-50 mg/L with a correlation coefficient of 0.9999. The limit of detection was 0.02 mg/L,0.01 mg/L and 0.01 mg/L respectively. The average recovery of three estrogens ranged from 85.6% to 106.3%. RSDs were 2.0%-4.8%. |
