Research On Phytic Acid Hydrolysis And The Products Of Separation

Raw material of production of phytic acid is abundance, of low cost and reproducible. In recent years, the researching area of phytic acid has expanded more. Except for being used directly, it can be utilized to convert into hydrolysis products or derivatives. In which, hydrolysis products of phytic acid possess unique physiological functions, and they are important in medical science. However, the preparation of hydrolysis products of phytic acid is rather difficult at present. The price of commodity form of hydrolysis products of phytic acid is very high commercially, which limits the application of them severely. Because of its versatile characteristics, the content of phytic acid has to be measured frequently in manufactures and studies. At present, there are various methods for its measuring and have different ways, since every method has its own advantages and disadvantages, the results may be inconsistent with each other, even very different when testing the same sample,so it will be very difficult for us to choose the proper means rapidly and accurately to determine phytic acid。This article focused on the factors influencing the hydrolysis process and species of hydrolysis products, such as temperature, time, concentration and acidity, and the methods of how to separate hydrolysis products. The investigation showed that the optimal conditions of hydrolysis required a temperature of 130 ?C, concentration of 10% and solid superacid as the catalyst. Under these conditions, When the hydrolysis products were mainly IP5 and phosphoric acid, the reaction time was about 2 h; When the hydrolysis products were mainly inositol pentaphosphate and phosphoric acid, the reaction time was about 5 h;when the hydrolysis products were mainly inositol triphosphate and phosphoric acid, the reaction time was about 8 h; When the hydrolysis products were mainly inositol biphosphate and phosphoric acid, the reaction time was about 11 h; When the hydrolysis products were mainly inositol phosphate and phosphoric acid, the reaction time was about 14 h. The hydrolysis products were separated and purified by the methods of anhydrous ethanol, inorganic precipitation and synthesis of inositol polyphosphate aminium salt, which was not the same as reported. The removal rate of inorganic phosphor by the method of anhydrous ethanol is about 80%, by the other two methods, they were both more than 92%.In this article, a spectrophotometry for measuring the phosphorus was improved,and was applied to determing phytic acid and compared with other three methods: The results indicated that among the four methods, the improved spectrophotometry for measuring the phosphorus was highest in precision, which was suited for determining the micro amount of phytic acid with no standards; which was followed by the spectrophotometric colorimeter for determining Fe, for the micro amount of phytic acid with standards; besides it was titration of thorium nitrate, for the rapid measurement for the high content of phytic acid; at last was indirect titration of thorium nitrate, for determining the high content of phytic acid roughly.