Synthesis And Spectral Properties Of Metal Complexes Of Nickel, Cobalt, Terbium Based On EDA

In recent years, metal-organic coordination compounds have been widely applied in the research fields of adsorption, catalysis, luminescence, electric, magnetism, owing to the merits of structure diversity, controllability, good thermal stability etc. Moreover, metal complexes extensively exist in organism and play a crucial role, and were often applied in pharmaceutical engineering. So, the research of structures and biological activities of metal complexes has become a new hotspot in chemistry field. In addition, the important informations of chemical substances on the transportation, distribution and metabolic processes in the body were obtained by studying on the interactions of small organic molecules, metal complexes with protein. It has great significance of drug screening and the research on pharmacology, toxicology and the bane mechanism of pollution toxicants. The main research work of this thesis is as follows:1. EDA was synthesized and the paleyellow crystals were obtained. The crystal structure was determined by X-ray diffraction method. The aqueous solution chemistry of EDA has been examined by using UV-Vis and fluorescence spectra at different pH and solvent.2. The interactions of different metal ions with EDA were studied by using UV-Vis and fluorescence spectra in 0.05M Tris-HCl buffer (pH 7.4). It can be drawn that EDA has highly selective recognition for Ni2+ over other various metal ions. Furthermore, nickel can form stable complex with EDA. The binding of nickel makes the fluorescence intensity of EDA be enhanced 1.8-fold. Morover, [Ni4(EDA)4]·2CH3CH2OH·9H2O was synthesized and the blue crystals were obtained. It can be concluded that nickel can form stable complex with EDA by 1:1 ratio in solution and solid.3. The interactions of EDA, Ni-EDA with BSA were studied by spectroscopic methods under simulatively physiological conditions. The binding constants and numbers of binding sites of EDA, Ni-EDA with BSA were obtained by fluorescence spectra. The thermodynamic parameters of the interactions of them were measured according to the van's Hoff equation. The data indicated that the interaction between EDA and BSA was driven mainly by hydrophobic interaction. The data indicated that the interaction between Ni-EDA and BSA was driven mainly by van der Waals force and hydrogen bonds. The binding distance of EDA from the tryptophan residue in BSA was calculated to be 2.14 nm based on the Forster theory of non-radiation energy transfer.4. The compound [Co(EDA)(py)2]Cl·3.5H2O was synthesized. The crystal structure was determined by X-ray diffraction method. In this compound, each Co (Ⅲ) center is six-coordinated and exhibits distorted octahedral geometry.5. The binding properties of EDA with terbium ion have been investigated by using fluorescence spectra. The fluorescence intensity at 545 nm was enhanced about 8-fold. The conditional binding constant was calculated to be K2Tb-EDA= 4.14×107 M-1. In addition, the compound K3[Tb2(EDA)4] Cl·CH3OH·3H2O was synthesized.