Studies On Extraction And Exploratory Development Of The Essential Oil From Four Perfume Plant

Sage,(?)emon balm,true tarragon and hyssop served as fragrance vegetables or acrid materials at ancient times.Now we can refine essential oil from them,manufactur floral water and medicine.Experiment results are as follows:1.Fragrance components are extracted by vapor extractio and SFE-CO₂ Results manifestation: extraction percentage of SFE-CO₂ is significant higher than using vapor extractio;comparison by extraction percentage,true tarragon＞sage＞lemon balm＞hyssop.Orthogonal experiment of SFE-CO₂ from true tarragon showing the best extraction technology is Pressure33Mpa, Temperature45℃,Time 1.0h.2.Essential oils are expertised by GC-MS.Sage is expertised 74 kinds of composition,main chemical compositions are 2,6-Octadienal,3,7-dimethyl-,(E),(relative amount is 22.39%), 2,6-Octadienal,3,7-dimethyl-,(Z),(relative amount is 11.91%),6-Methyl-1,5-heptadiene, (relative amount is 7.11%),2-Pentene,3-methyl-,(Z)-,(relative amount is 6.74%),Thujone, (relative amount is 5.74%).Lemon balm is expertised 67 kinds of composition, caryophyllene,.beta.-Pinene,D-Limonene,eucalyptol,and so on.True tarragon is expertised 74 kinds of composition,main chemical compositions is isoelemicin,(relative amount is 46.68%). Hyssop is expertised 75 kinds of composition,main chemical compositions are Cyclohexanone, 5-methyl-2-(1-methyl ethylidene)-,(relative amount is21.60%),Cyclohexanone,5-methyl-2-(1-methylethyl)-,trans-,(relative amount is13.20%),pulegone,(relative amount is7.03%), Phytol,(relative amount is5.03%),Germacrene D,(relative amount is4.59%),1,6-Octadien-3-ol, 3,7-dimethyl-,(relative amount is 4.38%),Cyclohexanol,5-methyl-2-(1-methylethyl)-,[IS-(1. alpha.,2.beta.,5.beta.)]-,(relative amount is 4.31%),and so on.3.Experimental results of bacteriostasis are as follows:inhibition ratio of colibacillus at high density,sage＞true tarragon＞hyssop＞lemon balm;inhibition ratio of staphylococcus aureus, sage＞true tarragon＞lemon balm＞hyssop;inhibition ratio of bakers' yeast,sage＞true tarragon＞lemon balm＞hyssop;inhibition ratio of aspergillus flavus,lemon balm＞sage＞hyssop＞true tarragon.