Analysis In Sophora Japonica L, Etc By Gas Chromatography/Mass Spectrometry

In this thesis, at first, gas chromatography/mass spectrometry (GC/MS) technology and its applications in various fields, its analysis characteristics,equipment configurations and relevant basal knowledge have been introduced. As one of the most important means—GC/MS has been applied extensively, the thesis summarized its applications in the four areas, namely food security,natural products,environmental protection and the court science.The main content has been divided into three parts. In first part, the chemical components in Sophora japonica L were analyzed by GC/MS. We focused attention on the analysis of the volatile oil and fatty acid components in Sophora japonica L. The samples processing and GC/MS conditions choice were studied. As a result, 30 components of the volatile oil were identified, which represented 84.41% of the total GC peaks of the volatile oil; 13 kinds of fatty acids were identified, and similarity of 13 species fatty acids all were more than 90%.In second part Caffeine in tea beverage was measured from the qualitative analysis and quantitative analysis with GC/MS. In qualitative analysis, the key step was sample preparation and the GC/MS analysis conditions choice. The qualitative method was compared with the caffeine standard spectrum. In quantitative analysis, the external standard method has been used to determine the caffeine concentration in tea beverage, using automatic sampling to solve the accurate sample volume in the external standard method. MS scan mode included Full-scan and SIM scan choice, in SIM scan, m/z 67,82,109 and 194 four ions was selected. Qualitative results were accurate, and standard spectrum similarity was 99 percent. Quantitative method was feasible, the recovery rate and precision were acceptable.Finally, the fatty acids of the sea cucumber extract was analyzed by GC/MS. The best experimental conditions were found. As a result, 31 kinds of fatty acids were identified. The components of fatty acids represent 80.33 % of the total fraction...