Modification And Compound Of Poly-ε-lysine Used As Tissue Engineering Scaffolds

With the development of tissue engineering, research on scaffold materials becomes more and more important. The exploitation of composite scaffolds with superior properties is now the hotspot in this field. Poly-ε-lysine (ε-PL) has the characteristics of nontoxic, virus-resistance, biodegradability, and can also accelerate the growth and adhesion of cells as well as promotes the cells to performance its normal function. But the disadvantages of low strength and brittleness restrain the applications as scaffold materials in tissue engineering. As a commonly used biomaterial, chitosan (CS) has strong toughness, good glutinosity and nontoxic, it can also speedup wound concrescence with minimal foreign body response. Bacterial cellulose (BC) is a kind of natural organic polymer with good mechanical properties and biocompatibility. Hydroxyapatite (HA) is an artificial substitute material in bone tissue engineering because of its perfect biocompatibility.In this paper, research on modification ofε-PL has been studied by the methods of crosslink with glutaraldehyde(GTA) and combination with CS. Modifiedε-PL andε-PL/CS were prepared and followed by freeze-drying with the purpose of obtaining porous structure. XRD, FTIR, and SEM were employed to investigate the microstructure and morphology of the composites. FTIR results indicate that chemical reaction took place during modification. XRD results confirmed the reaction by the change of crystal structure and crystallinity. SEM shows the microporous structure which could be controlled.In addition, carboxyl groups on BC surface were substituted by hydroxyl through modification, andε-PL/BC composites were obtained with modified BC. Furthermore, a mimetic route was used to prepare theε-PL/BC/HA composites. XRD, FTIR, SEM analyses were employed to investigate the microstructure and morphology of the composites. SEM pictures showed that HA pellet particles distributed on the surface of BC fibre, the amount of deposites increased as time increased.