One-step Synthesis To Polysucrose-based Nanoparticles

A self-assembly method was employed to synthesize water-soluble polysucrose-based nanoparticles by one step. Polysurose and acrylic acid (AA) worked as raw materials, and Cerium (IV) was used to initiate active spots onto polysucruse chains. AA was added onto polysucrose as graft-chains. Under acid condition, the nano-micelles were formed by self-assembly. Then the further addition of bi-functional cross-linker Methylenedioxymethamphetamine acrylamide (MBA) cross-linked the Poly (acrylic acid) (PAA) chains to fix the nano-micelles.Transmission electron microscope (TEM) indicated that the diameter of these nanoparticles was approximate 70 nm. IR, 13C NMR results confirmed the formation of resultant nanoparticles. The effect of reagent ratio on nanoparticles'size and diameter polydisperse index (PDI) in water was determined by Dynamics Light Scattering (DLS). From the results, the increase of AA content resulted in the increase of the dimension of resultant nanoparticles (from 209.7 nm to 293 nm) on condition that the content of other reactants were kept constant. And the PDI changed from 1.0391 to 1.0315, which indicated that the dispersion of the nanoparticles'effective diameter was very narrow in water; while the increase of initiator content resulted in the decrease of the dimension of resultant nanoparticles (from 244 nm to 281 nm) on condition that the content of other reactants were kept constant, which means the effect of initiator to the diameter was not more significant than the effect of AA. However, the PDI increased from 1.0315 to 1.0432.Bovine serum albumin (BSA) was chosen as protein model to study the protein adsorption capability of there nanoparticles. The effect of reagent ratio on this adorption capability was investigated. In addition, we also found that the protein absorption ability of the nanoparticles had a relationship with pH value of the synthesis system. The best loading efficiency was 13.6% and the lowest was 3.0%.