The Reasearch Of Special Lipase For Biodiesel

Biodiesel is a renewable, biodegradable, nontoxic and cleaning fuel, which can partly be substituted for conventional fossil diesel. Compared with the traditional alkali and acid catalysis on the synthesis of biodiesel, the enzymatic one has some advantages such as mild condition, no wastewater and easily separating the reactants.In this thesis, firstly, filter the lipase which is enzymatic synthesis of biodiesel and comparisons of transesterification of palm oil to biodiesel catalyzed by three different lipases of enzymatic synthesis of biodiesel and lipase which is enzymatic synthesis of biodiesel by former investigators. Find that PB011 lipase have upper catalyse trait and then through single factor designing experiment, the effects of temperature, enzyme dosage, addition times, solvent amount, substrate molar ratio, and water amount on percentage weight conversion to oil methyl ester were studied and the component of product was analyzed. The results show that the fitting reaction conditions for the PB011 lipase catalysing the soybean oil are 35℃, 10 % of enzyme dosage (relative to oil quality), addition for 3 times, solvent amount was 30mL and water amount was 0% (relative to oil quality), 1.5:1 of substrate molar ratio .The conversion rate can reach 93.21%.The production of enzymatic biodiesels through PB011 lipase catalyzed chemical reaction from soybean oil was optimized by the respons surface method (RSM). The reaction conditions affacted the biodiesels production were studied using the CCRD method of six factors and five levels, the optimal operate parameters and mainly effect factors were confirmed.The results showed the optimum reaction conditions were: 11.5% of enzyme dosage, 37.5℃, and addition for 4 times, 19mL solvent amount, 2.5% water amount, 1:1 of substrate molar ratio. Under those conditions, the conversion ratio can reach 96.89%.According to selecticity of lipase for oils, the production of enzymatic biodiesels through PB012 composite lipase catalyzed chemical reaction from soybean oil, palm oil, peanut oil and colza oil was optimized with variou of peculiar theories. The effect of enzyme amount, temperature, solvent, inhibition of alcohol, water content, molar ratio of substrate on esterification were studied in detail. The result show that, the production of enzymatic biodiesels from soybean oil,, the conversion rate can reach 97.58 %; the production of enzymatic biodiesels from palm oil, the conversion rate can reach 96.40 %; the production of enzymatic biodiesels from peanut oil, the conversion rate can reach 98.20%; the production of enzymatic biodiesels from colza oil, the conversion rate can reach 93.54 %. It is obvious that PB012 composite lipase have an upper convertion rate when it catalyses various axunge. To a certain extent, it eliminate the problem of limited material and open out a new specific lipase for biodiesel and lay the academic foundation for the industry of biodiesel, which is synthesized by the catalysed enzymic method.