Synthesize And Quality Control Of Dexrazoxane

OBJECTIVE: Cell protective agent Dexrazoxane has been successfully used prevents or reduces the heart toxicity of doxorubicin. It was synthesised first time in 1976 by Creighton. This paper aims to simplify the synthesis process of Dexrazoxane , and do preliminary research on quality of the final product.METHODS: Resolute the DL-1, 2-diaminopropane which been used as the starting material by D- (-)-tartaric acid. Then react with both formaldehyde and sodium cyanide after condensation to 1,2-diamino- -prpane tetracetonitrile, and then hydrolyze to (s)-(+)-N, N, N', N'- tetra- -caboxamindomethyl-1,2-diominopropane, at last change into dexrazoxane through cyclization reaction.RESULTS AND CONCLUSION: Target compounds'chemical structure was confirmed by IR, MS, HPLC, and the yield is 56.3%. We inspected dexrazoxane's solubility, melting point, and optical rotation. All of them are conform to the literature. At the same time we initially established the quality Standards of Dexrazoxane through non-aqueous titration, UV-visible spectrophotometer and HPLC. The experiments show that this route is simple and efficiency.