Isolation Of Dehydroabietic Acid And Its Solubility Measurement

Dehydroabietic acid was the main component of the disproportionated rosin, it was applied to medicine,pesticide,surfactant and the preparation of fine chemicals etc.due to their wide range of versatile,significant bioactivities as antibacterial,antivirus,anticancer,anti-ulcer.However,because the resin acid had similar chemical structures and it was very hard to isolate the monosome resin acid with the physical method.Therefore,it was only applied to the industrial production as a mixture which limited its application in other fields. This paper can increase its economic value and application fields in improving the exploitation of the rosin acids and its modified products.This paper covers the contents as follows:The materials and products were analyzed by gas chromatograph,using BP×5 boned phase,fused silica capillary column(30 m×0.32 mm×0.25μm),the gas chromatography conditions have been considered,the pressure before column was 0.07MPa.The flow rates of hydrogen gas and air were 30mL/min and 300mL/min,respectively.Nitrogen was used as gas carrier,with a flow rate of 45mL/min,and the plit flow ratio was set at 50:1.The flow rate of tail gas was 24mL/min,temperature of check machine was 533K,the temperature of injector and detector was 533K,the amount of injected material was 1.0μL.The temperature program was as follows:423K for 0 min,then 30K/min to 483K, and finally 2 K/min to 523K.In the comparison of methods for isolation of dehydroabietic acid,the comparison of methods for isolation of dehydroabietic acid was carried out, using disproportionated rosin as raw material and GC as the analytic method. Dehydroabietic acid was isolated from disproportionated rosin by methods of amination,saponification and aminating reaction-crystallization enhanced with ultrasonic wave respectively and the studies of isolating dehydroabietic acid from different kinds of disproportionated rosins have been investigated.The results showed that the method of aminating reaction-crystallization enhanced with ultrasonic wave was the best to isolate the dehydroabietic acid from the self-made disproportionated rosin at the temperature of 40℃,and the purity of dehydroabietic acid prepared was 99.99%,the yield of dehydroabietic acid was 55.37%.The high purity dehydroabietic acid prepared have been analyzed and confirmed by GC,UV,IR,melting point instrument and polarimeter as well,and the results are in agreement with the values in literature.The solubilities of dehydroabietic acid in four solvents of absolute ethanol, 95%ethanol,85%ethanol,and 75%ethanol were investigated at the temperatures from 283.60K to 324.25K with synthesis method and using the digital constant temperature bath.These data were not reported in other documents.