Molecular Toxicology Of Deltamethrin To Carassius Auratus

Deltamethrin, a predominant class of synthesized Pyrethroid, is highly bio-available and relatively low toxic to organisms compared with organochlorine and organophosphorous pesticides. It is used worldwide for pest control in households, cereals, vegetables, cotton with the development of chemical industry. However, its toxicity to non-target organisms is unknown. When it is applied to the soil and washed into the water body by rain-off, the water may be contaminated and become unsafe for drinking and other household purposes, also not for irrigation and fishery, nevertheless, it may affect the aquatic organisms(non-target) and endanger the aquatic ecosystem.In this study, the acute toxicity of Deltamethrin to the Crucian was carried out by using the static test system. The changes of serum hematological parameters, total esterase and carboxylesterase activities of liver, esterase isoenzyme and DPC in liver and kidney are studied by exposing Crucian to different Deltamethrin concentrations.According to the acute toxic experiment, the the 96hLC₅₀ of Deltamethrin for Crucian is determined to be 21.6μg/L. Thus, three sub-lethal dose groups—4.32μ/L,8.64μg/L,12.96μg/L (which is 20%, 40%, 60% of 96hLC₅₀ respectively,), are selected to treat Crucian for 3d, 6d, 9d.With Deltamethrin concentration increasing and duration time extending, the concentration of serum protein and urea nitrogen content decrease significantly compared with the control. Whereas, different changes, which rise up first and then go down with increased Deltamethrin level, are observed in the serum total lipid content and the activities of liver total esterase and carboxylesterase, but they have no significant difference as duration time prolongs. It indicates the Deltamethrin can affect protein and lipid metabolism in Crucian and cause damage to liver and kidney.Significant changes are also observed in four tissues' (liver, kidney, muscle, branchia) esterase isoenzyme electrophoretic zymogram carried out by using 10% polyacrylamide gel electrophoresis. The isoenzyme patterns of liver and kidney change much more than that of the muscle and branchia. As Deltamethrin concentration increases and exposing time prolongs, the EST6 in liver and EST3 in kidney disappear while the EST1 in kidney displays for a short period, indicating these two tissues are affected more seriously.The Deltamethrin can also induce DPC in the liver and kidney. As Deltamethrin concentration increases and exposing time prolongs, the DPC rates rise significantly in the kidney, while in liver it only shows in higher Deltamethrin concentration.