Research On Biotransformation Equipment Based On Immobilized Cells Of Producing Esterase

The immobilized method, cultivating conditions, splitting capability of the microorganism of producing esterase which splits D,L-lactic acetate were studied systematically in this paper. At the same time structure parameters were optimally designed considering the broad application of immobility bioreactor with few researches in this field, and a new-style immobility bioreactor was also designed, which was conveniently operated and easily invested.Equality-design to regression analysis was adopted for the sake of comprehending the effects of parameters on fermentation. The result of integrated consideration showed aerates volume affected oxygenic transfer and substance transfer badly, and viscosity coefficient took second place. Loading volume, immobility cells concentration and the pressure in the pot also affected differently.A new-style immobility cell bioreactor was designed in this paper. Air nozzle, sieve plate were designed mainly on base of the effects on oxygenic transfer and substance transfer, and the diameter of air nozzle is 2mm.A interior equipment with pinheads whose size is 16 to immobilize cells was designed aimed at the experiment request.An available and handy immobility model was set up by experiment. Spores were immobilized by calcium alginate and PVA and then were fermented. The optimal immobility condition that was gotten was calcium alginate3%, PVA8%,CaCl23%. Otherwise , the optimal reaction condition was T45C,pH6.5,3h.Immobility cells were fermented in air-lift bioreactor, at the same time feeling out the growth curve, remnant sugar and pH-variety curve. Subsequently the ending of immobilized cells and dissociate cells was compared. We found that the fermentation cycle of the immobilized cells in ALR shortened 20 hours than the dissociate cells in vase, and shortened 12 hours than the dissociate cells in agitation bioreactor.The splitting capability of immobility cells was studied after the offspring had been separated by ion-exchange and resin-adsorption. The result indicated that the purity of L-lactic acid achieved 84.9%.