Studies On The Preparation Process And Quality Standard Of Effective Component In Fritillaria

The dried bulb of Frritillaira, as a usual Chinese medicine is registered in Pharmacopoeia of People's Republic of China(2005 edition)volum 1. The Frritillaira has the glorious use history in China. Frritillaira has alleviates fever Run the lung, reduces phlegm and stops coughing effect. Mainly treats the lung hotly to cough dry, the expectorate belt blood, the phlegm multi-chests stuffily and so on sickness.An optimum method was established in this paper to extract and purify the effective fraction in Frritillaira. Adopting the method of orthogonal test and using the orthogonal table of L9( 3⁴), take the content of total alkaloid and the ratio of dry extraction as target, investigating the effect of extractive time, extractive number of times, fluid concentration on the extraction results, and then the optimum conditions for the extraction of total alkaloid was determined, which was adding 40% fluid, refluxing and extracting for 24h, totally extracting 2 times. Adopting the method of supercritical CO₂ fluid extraction technology extraction Frritillaira dissociation alkaloid, the selection extract pressure, the extract temperature, the extract time and the entrainer amount used carries on the single factor experiment as the inspection condition, in single factor experiment foundation, again through orthogonal test. The optimum conditions for the extraction of total alkaloid was determined, which was: extract pressure 20MPa, ethyl alcohol amount used 300ml, extract time 2h, extract temperature 45℃; Uses the ethyl alcohol (methyl alcohol) the rope type, the ether (acetone) the precipitation method extraction Frritillaira total sopanin. Adopting the method of orthogonal test and using the orthogonal table of L9( 3⁴), investigating the effect of alcohol concentration, alcohol amount, extractive time on the extraction results,and then the optimum extraction condition for the extraction time is 8 hours, the granularity is 100 items, the methyl alcohol density 100%.This paper has also carried on the discussion to Frritillaira 's quality specification, has determined the trace element of Frritillaira separately. Uses the method of DAD-HPLC preliminary construction Frritillaira 's fingerprint. And uses the molecular biology labeling technique exploring Frritillaira 's quality specification. Uses the method of ASS to determine 16 kind of trace elements from 10 different of samples. Take determines the result content as the target, the extraction characteristic factor, makes the cluster analysis using the characteristic factor. The result indicates the trace element, has the relevance with the traditional Chinese medicine quality specification. The experiment uses the method of DAD-HPLC to examines its principal constituent alkaloid to appraise its quality. Take the permine as the standard, uses the external standard method, and carries on processing of derivative to 8 kind of Frritillaira, examines in the alkaloid and makes the analysis. This research has established the Frritillaira fingerprint atlas initially, provides certain reference for the its appraisal and the quality rating; The experiment uses the method of RAPD to increase the total DNA of samples, and obtains DNA fragment atlas. In the identical position, the duplicated DNA banding "has" records as "1", "does not have" to record as "0", then carries on the analysis on the Rapdistance software, and cluster. Obtains the cluster chart had basically reflected these Frritillaira's sibship, indicated from this, the RAPD method may be able to appraise Frritillaira's quality specification.