Studies On The Purification And Application Of Konjac Glucomannan

Amorphophallus konjac is a monoecious and perennial herbaceous plant of Araceae.There are about 170 varieties in the world,and at least 21 varieties found in our country.There are mainly two varieties including Amorphophallus Albus and Amorphophallus Rivieri,in planting.The main component in the Amorphophallus konjac is konjac glucomannan(KGM),which has the function of shedding toxin,losing weight,relaxing bowels,cleaning stomach,controlling disease,diabetic and carcinoma,lowering blood-lipid and cholesterol,keeping salt-balance,adjusting immune function, regulating lipid metabolism,supplementing calcium and so on.Amorphophallus konjac was used as raw material in our experiment.The determination method of KGM,optimized conditions for purification of konjac glucomannan,and possibility for KGM to replace PAM were studied.The results were as follows:1.Wavelength of determing KGM by spectrophotometer was detected.The results showed that: the maximum absorption band was 497nm,under this wavelength,the Relative error of testing is 0.3%.and under wavelength of 550nm,the counterparts are 2.1%,respectively,and under wavelength of 550nm the relative standard deviatetion of veficity is 1.13%。2.It is feasible to purify the konjac glucomannan using Ethanol,and the optimized conditions were as follows:elution time 90rain,pH of the solution 4.5,concentration of ethanol 60%.Under those conditions,the maximum contents of KGM are higher up to 98.51%.3.The basic properties between highly purified KGM and PAM were caomapred.The results showed that it was almost same in the solubility in between KGM and PAM,except a little bit more dissolving in benzene for KGM,but no for PAM.The similar transparency existed between the two but slightly better for PAM.Meanwhile,the sound stability for both temperature and pH value was observed.