| Nowadays, organic acid, organic salts and organic ester are the main adoptiveantiseptics products in the world markets. They have some disadvantages such asbadness smell, thrill, causticity, carcinogenicity, teratogenicity, toxicity and non—degradable, which affect the security and edibility of food and feed, and areharmful to our health and environment hugely, though the anti-fungal effects werenice. Along with the food and feed safety attaching more emphasis, the studyconcerning about safe , low-noxious antiseptics has been becoming the hotspot andemphases of the development of food and feed.Litsea cubeba is a kind of safe, low poisonous, natural product, the principalconstituent of which was citral with excellent antibacterial effect, but it is difficult tomaintain long-term antibacterial effect due to strong volatility. The aim of the studywas to prepare safe, effective microencapsulation antiseptics using porous starch aswall martial, which were made of raw material starch, deproteinized starch, delipidstarch and both removal starch respectively byα-amylase(αAMY)and complexity ofα-amylase(αAMY) andα-1,4-glucan glucohydrolase(QAMY), the results showed thatdifferent kinds of porous starch products were successfully prepared ,which had beenidentified using Scanning electron microscope. The anti-fungal effect of litsea cubebaoil embedded by porous starch was studied. The results were as follows:1. To explore the optimum techniques for preparing microporous starch fabric-cated with unpolished rice and corn, four kinds of different dispose of material werestudied by usingα-amylase. The results showed that the optimal technical parametersof fabricating the unpolished rice porous starch granules.: amount of enzyme was 2.5mL, reaction time was 24h, pH was 5.5, temperature was 55℃;.the optimal technical parameters of fabricating the corn porous starch, granule: amount of enzyme was 2.5mL, reaction time was 28h, pH was 6.0, temperature was 55℃.In the optimalconditions of the experimentations, the adsorption oil ratio of the unpolished rice andcorn porous starch is respectively increased by 144.6 % and 204.8 % comparing tothe raw starch.2.In order to fabricate microporous starch which had higher adsorption oil ratio.the optimum techniques of preparing for microporous starch were studied from thedifferent disposed starch of unpolished rice and corn by usingα-amylase andα-1,4-glucan glucohydrolase. The results showed that the optimal technology parameters offabricating the unpolished rice porous starch granule.: reaction time was 24 h, pH5.5,temperature 50℃, starch concentration 50 %,α-amylase:α-1,4-glucan glucohy-drolase=4:1, the content of enzyme 55 %; the optimal technology parameters offabricating the the corn porous starch, granules: reaction time 28h, pH5.5, temperature55℃, starch concentration 50%, a-amylase:α-1,4-glucan glucohydrolase=8: 1, thecontent of enzyme 55 %. In the optimal conditions of the experimentations, the adsorp-tion oil ratio of the unpolished rice and corn porous starch respectively increased by216.0 % and 261.6 % comparing to the raw starch.The absorption oil ratio of all the 4 kinds of porous starch were increasedsignificantly by using complexity ofα-amylase andα-1,4-glucan glucohydrolase,The highest absorption oil ratio was 60.3 % when the porous was made of starchwith both protein and lipids removal. The highest comparative adsorption oil ratiowas 373.0 % when corn porous starch was made of starch with both protein and lipidsremoval. The absorption oil ratio of porous starch made of starch with protein andlipids removal increased significantly compared that of porous starch made of rawstarch.3 To explore the technology of fabricating microencapsulation of Listea Cubeba Oil,took the litsea cubeba oil embedded by the unpolished rice porous starch with bothprotein and lipids removal. Through verifying repeated experimentations of threetimes, the results showed that the rate of the litsea cubeba oil embedded by porousstarch (litsea cubeba oil microencapsule) was 29.2 percent,the content of litsea cubeba oil litsea in cubeba oil microencapsule was 21.3±0.2 mg/g under t the optimaltechnology parameters of encapsulating temperature of 55℃,encapsulating time of 1 hand w(wall material) : w(core material)=3 : 1.4 .The results of anticorrosion experiment indicated that cubeb oil microencapsulehad better effect of inhibiting mold than litsea cubeba oil and nisin with the sameamount, and had similar effect of inhibiting bacterium with potassium sorbate; butlitsea cubeba oil and microencapsulation of Listea Cubeba Oil had better effects of inhibitingmold spore than potassium sorbate.5.The results of anticorrosion experiment of microencapsulation of Listea Cubeba Oilindicated the cubeb oil microencapsule has the sustained-release effect to some extent,and had better effect of inhibiting mold than nisin,litsea cubeba oil and potassiumsorbate. |
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