Application Study On Standard For The Quality Control On Vitacoenzyme, Pamabrom And Compound Tetrazin And Reserpine Using HPLC

Along with the chromatograph theory and the technical unceasing consummation and the development, high performance liquid chromatography (HPLC) is grdually popullar in the medicine research and the development use. Now it is the most powerful separation tool in the medicine purity appraisal and medicine metabolism studies. In recent years, not only along with the new highly effective fixed phase padding, the chromatographic column filling technology as well as the advanced self-reacting device development, HPLC took highly effective, keen, the fast analysis method uses in the medicine the initial period development and the daily quality control. Moreover as a kind of highly effective and ideal separation and purifies technology it is favored in chemistry and in the biological drugs manufacture industry.The Vitacoenzyme is one of significant achievement prize projects that Chinese Academy of Science attains and it is the Chinese domestic new medicine. In the world, there isn't the same variety and it is a totally new style. Moreover, it is a kind of medicine food homology new variety. The Compound Tetrazin and Reserpine Tablets is a kind of common bloodpressure medicine. In the pharmacopoeia of People's Republic of China, it is too simple to regulate its quality specification formulation. Therefore to formulates an more perfect quality specification is absolutely important. The Pamabrom raw material and its preparation ammonia phenol Pamabrom piece is a medicine which a kind of treatment Dysmenorrhea, the researches about its preparation quality specification haven't been reported. Therefore, it is extremely essential to formulate a simple effective quality specification for the Pamabrom and its preparation ammonia phenol Pamabrom piece.The essence this article studies includes three aspects:1. Separates the Vitamin B₂ in the Vitacoenzyme with high performance liquid chromatography. What's more, the method to measure the determination Vitamin B₂ content has been found. The result indicated that the effect of this method to determine Vitamin B₂ in the Vitacoenzyme is good. At the same time, it provides the effective reference to consummate the quality specification of Vitacoenzyme. 2.Established the method of the opposition highly effective liquid phase chromatography to examine the Pamabrom raw material and the Pamabrom content determination in the ammonia phenol Pamabrom piece. Finally it shows that the method to determines the Pamabrom and the preparation has high sensitivity and the determination scope inside the connection sexual relationgship is good and the result is stable. It has made the very good reference to study the Pamabrom and its the preparation ammoni aphenol Pamabrom quality specification.3.It has studied the method of highly effective coseparation in the liquid phase chromatography determination Compound Tetrazin and Reserpine Tablets Vitamin B₁, Vitamin B₆, Hdralzine Hydrochloride, Chlordiazepoxide, Rutin, the grass cutter five ingredient methods. The result showed the result of this method to determine five kinds ingredients of the Compound Tetrazin and Reserpine Tablets is stable and the effect is good.