The Enzymatic Preparation Of Sea Cucumber Peptide And Its Anti-fatigue Action

The thesis was about the enzymatic preparation of bioactive peptides from sea cucumber and its molecular weight distribution determined by ultra-filtration and HPLC, the anti-fatigue action of which was studied as well.Studies of enzymatic hydrolysis were mainly on the effect of pH, concentration of substrate, dosage of enzyme, hydrolysis time and temperature on the yield of polypeptide, and the crude sea cucumber peptide was made under the optimum hydrolytic condition. In order to multiply utilize the nutritional ingredients of sea cucumber, the polysaccharide was purified. The experimental result is as follows: the concentration of substrate is 1%, the dosage of protease is 8000U/g, temperature is 50℃and hydrolysis time is 2h. Under this condition, the yield of polypeptide is 34.26%. After separation and purification, the yield of polysaccharide is 0.402%(compared with fresh sea cucumber).Gel chromatography of molecular weight distribution was obtained by HPLC after the purification of sea cucumber peptide with ultra-filtration membrane. The results indicate that the active peptide has a similar molecular weight, which is less than 1600Da. The proportion whose molecular weight is less than 1000Da is 72.07%. The result showed that ultra-filtration can effectively remove the macromolecular protein of the hydrolysis production.The anti-fatigue effects of sea cucumber peptide on mice were studied through different dose. The results show that sea cucumber polypeptide have no obvious effect on body weight in mice, can significantly prolong the time of loaded-swimming and rolling stick, strongly degrade the content of blood urea nitrogen and increase the content of hepatic glycogen of post exercise mice. The sea cucumber peptide has effective actions of raising exercise tolerance, promoting glycogen reservation and accelerating the metabolism of urea nitrogen and the anti-fatigue action is enhanced by increasing the dosage.