Screening Of Nitrobenzene Degrading Strain And Studying On Its Application

The nitrobenzene is an important chemical industry material, which is used widely in manufacture aniline, dye, TNT, soap, spice and so on. It is faint yellow, with bitter almond taste poisonous matter, and hardly to be biodegraded. However, the demand of nitrobenzene show ascendant trend with the chemical industry development, thus bring heavy contamination to the water with the effluence. At present, the common technology in removal nitrobenzene from waste water includes physical chemistry methods, such as activated carbon adsorption, oxidation, extract, irradiation, and so on. These methods are high cost, and tending to cause second pollution. Although biodegradation is regarded as an economical and efficacious purified method in the treatment of industry waste water and sewage, the high toxicity of nitrobenzene would limit its application. Therefore, it is significant to screen bacterium that can effectively degenerate nitrobenzene and inoculate it in the nitrobenzene wastewater process project.In this paper, the screening, characteristics and application of the nitrobenzene degeneration bacterium were investigated. Through the domestication in feast/fast, two strains that could degenerate nitrobenzene effectively were screened, named as N1 and N7. When initial nitrobenzene concentration was 400ug/L, the degeneration rate of the two strains reached 62.2% and 61.5% respectively after 24h reaction. It's identified that both of the two strains were Klebsiella by the traditional and molecular biological technology. The researches showed that N1 strain with higher nitrobenzene degradation ability grew well in the condition that pH 6.5~7.5, temperature 25~35℃, shaker speed 160r/min. Adding certain quantity carbon source and phosphorus source would accelerate the growth of N1. And the bacteria growth increased with the increase of C/N when the ratio of C/N ranged from 5:1 to 20:1. There should be coordination degeneration function between N1 strain and the aniline degeneration strain. Moreover, N1 also had high degradation ability to the aniline. N1 strain has high cell-surface hydrophobicity (CSH). When it was in its logarithm time, the CSH of N1 was about 70%.