Primary Investigation The Biological Removal Efficiency Of Nitrobenzene Polluted Groundwater Sources By PRB

The earliest application of groundwater pollution repair technology is thetraditional method at home and abroad,such as isolation method, extraction-treatmentmethod, adsorption and electrochemical method and air blowing off method. But thetechnology has some limitations and unable to continue to handle the pollutants andarrive to the good treatment effect. Therefore, we must explore and develop asustainable and effectiveness of groundwater pollution repair technology incombination with the actual situations of the groundwater pollution.Since the1970’s, the bioremediation gradually run into people’s vision. Manycountries in the world have been conducted about the pollution of the groud waterbioremediation technology research, but the technology has the later start about therepair management technology in the groundwater pollution in China.Groundwater pollution repair technology mainly have biological shots, organicclay method, extraction-back-to-note method, biological reactor and so on.But thetechnologies have their own weakness. Biological shots method is limited bypollution sources,the rock-forming and the soil and usually is only applicable to thesoil gas lift technology of feasible places; organic clay method has specific aim andeasy to secondary pollution and has not the good effect; extraction-back-to-notemethod and biological reactor have certain limitations, need from the groundwaterpollution by pump, and the equipment engineering takes up the large area, and there isa “trailing effect” and “rebound effect”, the operation cost is high and can’t use for along time.Therefore, the topic applied the immobilized biological PRB technology whichrepair groundwater pollution, it was mainly that immobilization combined withbiological PRB technology so that avoid the defects of the sity groundwater repairtechnology of cast bacteria. Immobilized materials embedded strains into carrier.(1) Itcan solve the inhibition of the pollution source of toxic pollutants, strengthen theenvironmental impact resistant microbes ability.(2) It can make the higher microbialcontent in BPRB, strengthen the stability of microorganisms, and greatly improve the degradation of pollutants’ effect. The technology was no power, simple engineeringfacilities, low operating cost, thorough, and didn’t need to repeat. The main researchas follows.(1) The inhibit concentration of free bacteria was450mg/L, and the experimentprepared the polyvinyl alcohol phosphate immobilized biological material.In thesame biomass and initial concentration of nitrobenzene conditions, the effects ofnitrobenzene degradation by immobilized bacteria was significantly higher thanthat of free bacteria.(2) In the PPVA peatmoss bioactive materials, curve dynamics of nitrobenzenedegraded by immobilized degrading bacteria accorded with zero level kineticequation,and half-life were between59h and129h. The experiment studied theinfluence factors of nitrobenzene degradation by immobilized microbial bacterium,such as the initial investigation of nitrobenzene,temperature,phosphorus source,carbon cource, nitrogen source and so on.Various factors determined the optimumparameters of degradation. The experimental results showed out that immobilizednitrobenzene degradating bacterium were in a relativedly good nutrition statewhen the concentration of nitrobenzene were between300mg/L and750mg/L,and had the strong ability to degrading nitrobenzene. It belongs to cold-adaptedmicroorganisms at10℃. The immobilized microbial degradation bacteria had agood growth in the presence of P element, but not in the presence of non-P,C,Nsource.(3) The experiment of preparing water and pollution site’s raw water examined thestability of degradation by the phosphate PVA immobilized microorganism. Thestability test totally run42days,and the degradation rate of nitrobenzene was morethan60%. Peatmoss soil-PPVA immobilized microorganism materials have thedegradation ability with the raw water of Jilin chenmical plant Songhua riverpollution, the result showed out non-nitrobenzene but aniline.(4) Assemble and operate the simulation column, and run the tracer experiment todeterminating the flow velocity of groundwater for further development ofnitrobenzene migration rule and necessary parameters basis. Through the simulation experiments in the laboratory,migration behavior and the change ofDO and pH was investigated to reveal the migration rule.