| The regenerativeness biological resource can ben changed into protein forage under the role of microbe. It can solve protein resource shortage, make rubbing , broad forage source raises forage quality , promotes agriculture under being helpful for the condition of ecological balance can develop continuously; Tradition ferment technology have more and more been hard to satisfy modern biology ferment the needs of project.It force people to improve continuously by using the related subject and newest research to accomplish ferment technology.The mechanical biography quality role of Ultrasonic, heating role and cavitation role of Ultrasonic are relevant to the frequency and power of the utrasonic. Ultrasonic power is smaller than certain value, Ultrasonic can be larger than Ultrasonic for the catalysis role of Enzyme reaction as Enzyme lose alive role, can promote cellulose, Enzyme untie reaction; Low power Ultrasonic, can decrease to ferment, liquid the accumulation of raw supersesson outcome shorten for the supersesson inhibition of microorganism to postpone issue, decrease to ferment, time, promote microorganism, hyperplasia and grow up.This paper with the root in corn straw stalk department cut off for major raw material to dry( 105℃, 12 hours to constant weight) rear process plant pulverizer preliminary breakage, then, cut again via high speed, machine cut smash powder, powder is packed into jar, is spare.Under low power Ultrasonic condition, carry out cellulose for straw stalk powder, Enzyme untie and produce Ruan false silk yeast to ferment test , determine that the best echnology parameter of processing SCP under the low power Ultrasonic condition.Related conclusion and the specific research content of this paper are as follows:(1) Ordinary water bath and Ultrasonic water bath go on for the straw of colloid mill handling, sour handling, soda handling and such pretreatment way preliminary Enzyme untie experiment, pass through result trade off study: it is show that using colloid mill handling straw, stalk is more economic feasible pretreatment method; Low power Ultrasonic can raise the rate of reduced sugar.Under handling condition, though in corn straw stalk the most of semi-celluloses by degradation, wooden quality vegetarian meal retain still is remaining produce space in thing and for the adsorption of Enzyme cellulose to separate; It is rear that corn straw stalk is handled by soda, the most of semi-cellulose and wooden quality vegetarian meal had been taken off , exclude , since the crystallization of base thing reduces , porosity increases , makes C 1 Enzyme with CMC Enzyme easy adsorption goes to corn straw stalk dimension base thing; It is rear that corn straw stalk is handled by colloid mill, crystallization degree had been destroied , base thing pellet grows , it is Enzyme that surface product increase is helpful for adsorption cellulose more. So can reach conclusion: The Ultrasonic adopted by this paper cooperate with cellulose Enzyme Enzyme untie the method effect of exceeding the tiny corn straw stalk pellet that smashed is very ideal , and have reaction time is short , reaction condition is mild , solubility reduced sugar have to lead high advantage.(2) Cellulose Enzyme untie needs certain base thing density, from economic angle consideration, adding Enzyme quantity will also set bounds, this paper pass through firmly liquid than have to lead for reduced sugar influence , cellulose Enzyme density have determined corn straw stalk for the experiment of influence of the content of reduced sugar Enzyme untie best firmly liquid than( 1: 15) and best Enzyme density( 12ul/ml).(3) Pass through for Ultrasonic power for corn straw stalk Enzyme untie the analysis of single factor test result of influence, show to work as, Ultrasonic power is smaller than certain value ( test in best power for 10 ws), Ultrasonic can be larger than Ultrasonic for the catalysis role of Enzyme reaction as Enzyme lose alive role, under the ultrasonic working condition of not destroying Enzyme protein structure , Ultrasonic can promote the interaction between base thing member, strengthen reaction thing to enter and generate thing to leave the course of Enzyme active center; Ultrasonic can speed up biography quality, have accelerated the sport speed of solvent and outcome, make cellulose Enzyme, member spread cell wall more easily , speed up so, Enzyme untie cellulose. when power exceeds 10 ws, the content of reduced sugar begins to drop , explain cellulose, Enzyme under power higher condition, Enzyme active drop , Ultrasonic as Enzyme have produced damage to act on, as Enzyme lose alive role is larger than act on for Enzyme catalysis. (4) Acorrding to the 3 factors and 3 horizontals orthogonality test( select L9(34) orthogonality show ), which based on the affects of corn straw stalk Enzyme untie temperature , Enzyme untie pH and time for the sugar content of reduced sugar, have determined corn straw stalk powder under Ultrasonic condition, best Enzyme untie technology parameter: Ultrasonic frequency 20 kHz ,power 20 ws, proportion between solid and liquid is 1: 15, Enzyme quantity 16ul/ml, PH value 5.0, Enzyme untie temperature 40℃, Enzyme untie time120 mins. The content of reduced sugar is highest under this condition.(5)Basing on the analysing Enzyme untie reaction the foundation model principle, have established cellulose Enzyme Enzyme untie the dynamics model of corn straw stalk: nder condition of ultrasonic.Combine application least-square to beg, Acording to the Enzyme untie reaction the foundation model, we can get the Enzyme Enzyme untie dynamics model parameter. Through testing, we verify the value of actual measurement and the simulated value of dynamics model and the two values can coincide better.So it can be used to guide production.(6)Having explained the life campaign of Ultrasonic for cell from cell by the angle of power influence. Test for the single factor of Ultrasonic power, Ultrasonic power less , yeast dry weight increases with Ultrasonic power increase; when Ultrasonic power exceeds certain value, it is little that yeast dry weight reduce with Ultrasonic power increase; it can be explaned when Ultrasonic power have damage or inhibition for yeast ferments when the power is too high,. Ultrasonic power for 12 w, yeast dry weight reaches biggest value. So it is 12 wthat this paper chooses Ultrasonic power in the following experiment.(7) Acorrding to the 3 factors and 3 horizontals orthogonality test( select L9(34) orthogonality show ), which based on ferment time , ferment temperature and initial pH , it can be inferred that yeast dry weight as ferment time prolong increase , but 24 h with 28 hs ferment the yeast increase of dry weight in time is very little , from time cost, consider to select 24 hs for ferment time. In fermenting early stage, yeast dry weight increases along with the increase of temperature, when temperature increases continuously, yeast dry weight begins to drop. Evidently under Ultrasonic condition, produce Ruan false silk yeast to ferment, existent best temperature. From experiment best temperature value 30℃, is suitable to produce the training temperature of Ruan false silk yeast (30-32℃). Since testing, can find out in corn straw stalk, Enzyme untie liquid under the Ultrasonic condition of 20 kHz and 10ws, best training condition for training temperature 30℃, initial PH value is 5.0, ferment , 24 time hs.(8) In this testing, the growth temperature of Ruan false silk yeast is constant, and the supply of various nutrition materials is limited, and the outcome feedback restrains effect is existence, this belongs to the growth course under restricted condition. Handling the rising stage of growth curve with biological group limited increase equation accord with experiment result completely. So when yeast grow in the restricted condition, the instant yeast total and time relation of early stage satisfy type at the first of stage of growth: .Through joining equation, beg to untie parameter, .so, have established yeast growth model under the condition of Ultrasonic. |
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