Process Optimization For Simultaneous Purification Of PC And PE By Alumina Column Chromatography

High-purity phosphohpids are widely used in the fields of food, pharmacy, cosmetic and so on because of the special surface activity and emulsification. .At present most of this production is imported in China and the requirement enhances every year. So it is necessary to develop the preparation of high-purity phospholipids. The research has been doing and great process has been made in our lab.Aiming at the process optimization for simultaneous purification of PC and PE by alumina column chromatography, the effect of different elution phase on the elution characteristic of PC (Phosphatidylcholine) and PE (Phosphatidyl-ethanolamine) on aluminium oxide column was studied. The regeneration of alumina oxide and the contiunous loading of the process were experimented. The process was developed and optimized absolutely.The elution effect of chloroform-methanol, methanol-chloroform-triethylamine, methanol-ammonia and methanol-chloroform-ammonia on the process for simultaneous purification of PC and PE by alumina column chromatography was studied. The optimized proportion of elution phase is methanol: chloroform:ammonia = 80 ∶120∶10 (v/v) and under this condition the recoveries of PC and PEwere both beyond 98% and the theoretical output of PC and PE were 0.31g/g sorbent and 0.04 g/g sorbent.The regeneration and continous loading were experimented and under the condition of elution phase 80% chloroform-methanol the recoveries of PC and PE were 99.8% and 86.8%; the theoretical outputs were 0.32 g/g sorbent and 0.03 g/g sorbent, the second and third loading amount were as much as the 80.2% and 79.7% of the firet loading amount. PC and PE with purities over 95% and 80% could beobtained. Under the elution condition of methanol: chloroform: ammonia =80 ∶120 ∶10 (v/v), the second loading amount was 10.85g which was 94.7% of the fist loading amount.Based on the former research, the effect of the different elution phases on lecition stability was studied. Experimental results showed that PC and PE were unstable in the solvent with ammonia or triethylamine. The result was valuable for the following work and industrial practice.The process for simultaneous purification of PC and PE by alumina column chromatography was developed and optimized which has satisfied effect, simple techniques, less investment and large amount loading. The work will provide important basic data for industrial preparation of high-purity phospholipids with alumina column chromatography.