| In 1957, A. Isaacs and J. Lindenmann discovered virus interference phenomena for the first time. A cell infected by virus can produce a kind of factor, which influences other cells and interferes virus reproduction, and so this kind of factor is called interferon. Upon its discovery, the interferon was widely attached with importance thanks to its avirulence, broad spectrum effect, and weak antigenicity. As a whole, the result of using interferon to cure virosis and some malignant tumors is inspiring, and the curative effect is certainly undoubted. Human leucocyte interferon is a set of albumen reagent produced by the leucocyte of a normal person under the effect of attractant, and has various strong biological activities, such as specific anti-reproduction of virus, anti-growth of tumors, adjustment of organic immuno-reaction. By referring to Cantell method and ultrafiltration salt-out combination method introduced by Liu Yanchun, this design and production processing uses NDV F series as attractant virus. Based on looking through relative materials, the production processing conditions are screened and discussed boldly. The result simplifies production process, largely reduces purification period (from 6 days to 2-3 days), saves human and material resource, decreases cost, reduces pollutant, and improves quality.When producing interferon, the blood verdure degree is in direct ratio to interferon output. Therefore, the collected anti-cruor blood must be fresh, and it'll be better to bring the blood to the separated gray-yellow layer within 24 hours. The produced gray-yellow layer has a great deal of erythrocytes. The NDV used during attraction has hemolysis effect on erythrocytes, and... |
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